Toward Retinal Organoids in High-Throughput
- 22 May 2023
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Perspectives in Medicine
- Vol. 14 (3), a041275
- https://doi.org/10.1101/cshperspect.a041275
Abstract
Human retinal organoids recapitulate the cellular diversity, arrangement, gene expression, and functional aspects of the human retina. Protocols to generate human retinal organoids from pluripotent stem cells are typically labor intensive, include many manual handling steps, and the organoids need to be maintained for several months until they mature. To generate large numbers of human retinal organoids for therapy development and screening purposes, scaling up retinal organoid production, maintenance, and analysis is of utmost importance. In this review, we discuss strategies to increase the number of high-quality retinal organoids while reducing manual handling steps. We further review different approaches to analyze thousands of retinal organoids with currently available technologies and point to challenges that still await to be overcome both in culture and analysis of retinal organoids.Keywords
This publication has 89 references indexed in Scilit:
- Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene DeliveryPublished by Springer Science and Business Media LLC ,2022
- Toward the next generation of vascularized human neural organoidsMedicinal Research Reviews, 2022
- A look into retinal organoids: methods, analytical techniques, and applicationsCellular and Molecular Life Sciences, 2021
- Electrophysiological Characterization of Photoreceptor-Like Cells in Human Inducible Pluripotent Stem Cell-Derived Retinal Organoids During in Vitro MaturationThe International Journal of Cell Cloning, 2021
- Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem CellsCurrent Protocols in Immunology, 2020
- Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependentThe International Journal of Cell Cloning, 2019
- Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological DifferencesStem Cells Translational Medicine, 2019
- Human-Induced Pluripotent Stem Cells Generate Light Responsive Retinal Organoids with Variable and Nutrient-Dependent EfficiencyThe International Journal of Cell Cloning, 2018
- Generation of Storable Retinal Organoids and Retinal Pigmented Epithelium from Adherent Human iPS Cells in Xeno-Free and Feeder-Free ConditionsThe International Journal of Cell Cloning, 2017
- Optic Vesicle-like Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease TreatmentThe International Journal of Cell Cloning, 2011