A GoldenBraid cloning system for synthetic biology in social amoebae
Open Access
- 30 March 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 48 (8), 4139-4146
- https://doi.org/10.1093/nar/gkaa185
Abstract
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria–host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid.Funding Information
- National Institutes of Health (R35GM118016, R21OD022981)
This publication has 32 references indexed in Scilit:
- Examining multiple cellular pathways at once using multiplex hextuple luciferase assayingNature Communications, 2019
- FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biologyFungal Genetics and Biology, 2018
- GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological dataNucleic Acids Research, 2017
- A naturally monomeric infrared fluorescent protein for protein labeling in vivoNature Methods, 2015
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytesCellular and Molecular Life Sciences, 2014
- GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic BiologyPlant Physiology, 2013
- A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in DictyosteliumFEBS Letters, 2004
- Pleckstrin Homology Domain Diffusion in Dictyostelium Cytoplasm Studied Using Fluorescence Correlation SpectroscopyOnline Journal of Public Health Informatics, 2004
- Optimizing heterologous expression in Dictyostelium: importance of 5' codon adaptationNucleic Acids Research, 2000
- A defined minimal medium for axenic strains of Dictyostelium discoideumProceedings of the National Academy of Sciences of the United States of America, 1977