Molecular diagnostics in South Africa and challenges in the establishment of a molecular laboratory in developing countries
Open Access
- 31 March 2020
- journal article
- review article
- Published by Journal of Infection in Developing Countries in Journal of Infection in Developing Countries
- Vol. 14 (03), 236-243
- https://doi.org/10.3855/jidc.11779
Abstract
The laboratory plays a significant role in public health surveillance, outbreak investigation and infection prevention and control strategies. Microbiology laboratories are moving towards incorporating molecular biology techniques for the surveillance and identification of pathogens causing infectious diseases as well as the genotypic characterisation of these organisms. These methods are accurate, rapid, reliable, and provide a wealth of information that are not available using conventional phenotypic methods. However, establishing such a laboratory can be challenging in developing countries due to poor infrastructure, the lack of funding and the required expertise. This manuscript discusses the essential issues that need to be addressed when establishing a molecular microbiology laboratory and the usefulness of molecular techniques in public health surveillance and outbreaks in developing countries. Molecular data on South African findings obtained from surveillance and outbreak studies are also presented in this manuscript.Keywords
This publication has 24 references indexed in Scilit:
- Emerging Technologies for the Clinical Microbiology LaboratoryClinical Microbiology Reviews, 2014
- Whole-genome sequencing in health careEuropean Journal of Human Genetics, 2013
- Emergence of OXA-48 and OXA-181 Carbapenemases among Enterobacteriaceae in South Africa and Evidence of In Vivo Selection of Colistin Resistance as a Consequence of Selective Decontamination of the Gastrointestinal TractJournal of Clinical Microbiology, 2013
- Molecular techniques for detecting and typing of bacteria, advantages and application to foodborne pathogens isolated from ducks3 Biotech, 2012
- The dominant methicillin‐resistant Staphylococcus aureus clone from hospitals in Cape Town has an unusual genotype: ST612Clinical Microbiology & Infection, 2011
- Molecular Characterization of Clinical Methicillin-Resistant Staphylococcus aureus Isolates in South AfricaJournal of Clinical Microbiology, 2010
- Strengthening Laboratory Systems in Resource-Limited SettingsAmerican Journal of Clinical Pathology, 2010
- Emergence of Endemic Serogroup W135 Meningococcal Disease Associated with a High Mortality Rate in South AfricaClinical Infectious Diseases, 2008
- Application of Molecular Techniques to the Study of Hospital InfectionClinical Microbiology Reviews, 2006
- Enterobacterial Repetitive Intergenic Consensus (ERIC) Sequences in Escherichia coli: Evolution and Implications for ERIC-PCRMolecular Biology and Evolution, 2006