High‐throughput phenotyping accelerates the dissection of the dynamic genetic architecture of plant growth and yield improvement in rapeseed
Open Access
- 4 May 2020
- journal article
- research article
- Published by Wiley in Plant Biotechnology Journal
- Vol. 18 (11), 2345-2353
- https://doi.org/10.1111/pbi.13396
Abstract
Rapeseed is the second most important oil crop species and is widely cultivated worldwide. However, overcoming the “phenotyping bottleneck” has remained a significant challenge. A clear goal of high‐throughput phenotyping is to bridge the gap between genomics and phenomics. In addition, it is important to explore the dynamic genetic architecture underlying rapeseed plant growth and its contribution to final yield. In this work, a high‐throughput phenotyping facility was used to dynamically screen a rapeseed intervarietal substitution line population during two growing seasons. We developed an automatic image analysis pipeline to quantify 43 dynamic traits across multiple developmental stages, with 12 time points. The time‐resolved i‐traits could be extracted to reflect shoot growth and predict the final yield of rapeseed. Broad phenotypic variation and high heritability were observed for these i‐traits across all developmental stages. A total of 337 and 599 QTLs were identified, with 33.5% and 36.1% consistent QTLs for each trait across all 12 time points in the two growing seasons, respectively. Moreover, the QTLs responsible for yield indicators colocalized with those of final yield, potentially providing a new mechanism of yield regulation. Our results indicate that high‐throughput phenotyping can provide novel insights into the dynamic genetic architecture of rapeseed growth and final yield, which would be useful for future genetic improvements in rapeseed.Keywords
Funding Information
- National Natural Science Foundation of China (31770397)
This publication has 25 references indexed in Scilit:
- Genetic Properties of a Nested Association Mapping Population Constructed With Semi-Winter and Spring Oilseed RapesFrontiers in Plant Science, 2018
- Academic and Economic Importance of Brassica napus RapeseedPublished by Springer Science and Business Media LLC ,2018
- Genes and Quantitative Trait Loci Mapping for Major Agronomic Traits in Brassica napus L.Published by Springer Science and Business Media LLC ,2018
- Crop 3D—a LiDAR based platform for 3D high-throughput crop phenotypingScience China Life Sciences, 2017
- Assembly and comparison of two closely related Brassica napus genomesPlant Biotechnology Journal, 2017
- A high-density SNP genotyping array for Brassica napus and its ancestral diploid species based on optimised selection of single-locus markers in the allotetraploid genomeTheoretical and Applied Genetics, 2016
- Dissecting the Phenotypic Components of Crop Plant Growth and Drought Responses Based on High-Throughput Image AnalysisPlant Cell, 2014
- Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genomeScience, 2014
- Detection and genotyping of restriction fragment associated polymorphisms in polyploid crops with a pseudo-reference sequence: a case study in allotetraploid Brassica napusBMC Genomics, 2013
- A Multiple Comparison Procedure for Comparing Several Treatments with a ControlJournal of the American Statistical Association, 1955