Intramembranous bone regeneration and implant placement using mechanical femoral marrow ablation: rodent models
- 7 September 2016
- journal article
- Published by Portico in BoneKEy Reports
- Vol. 5, 837
- https://doi.org/10.1038/bonekey.2016.61
Abstract
In this paper, we provide a detailed protocol for a model of long bone mechanical marrow ablation in the rodent, including surgical procedure, anesthesia, and pre- and post-operative care. In addition, frequently used experimental end points are briefly discussed. This model was developed to study intramembranous bone regeneration following surgical disruption of the marrow contents of long bones. In this model, the timing of the appearance of bone formation and remodeling is well-characterized and therefore the model is well-suited to evaluate the in vivo effects of various agents which influence these processes. When biomaterials such as tissue engineering scaffolds or metal implants are placed in the medullary cavity after marrow ablation, end points relevant to tissue engineering and implant fixation can also be analyzed. By sharing a detailed protocol, we hope to improve inter-laboratory reproducibility.Keywords
This publication has 52 references indexed in Scilit:
- Mobilization of bone marrow mesenchymal stem cells in vivo augments bone healing in a mouse model of segmental bone defectBone, 2012
- Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized DefectsJournal of Visualized Experiments, 2012
- Bone turnover markers correlate with implant fixation in a rat model using LPS‐doped particles to induced implant looseningJournal of Biomedical Materials Research Part A, 2012
- The transcriptome of fracture healing defines mechanisms of coordination of skeletal and vascular development during endochondral bone formationJournal of Bone and Mineral Research, 2011
- Temporal Gene Expression Profiling during Rat Femoral Marrow Ablation-Induced Intramembranous Bone RegenerationPLOS ONE, 2010
- Testing the Critical Size in Calvarial Bone Defects: Revisiting the Concept of a Critical-Size DefectPlastic and Reconstructive Surgery, 2010
- Bone lengthening osteogenesis, a combination of intramembranous and endochondral ossification: an experimental study in sheepStrategies in Trauma and Limb Reconstruction, 2010
- Projections of Primary and Revision Hip and Knee Arthroplasty in the United States from 2005 to 2030The Journal of Bone & Joint Surgery, 2007
- Peri‐implant bone formation and implant integration strength of peptide‐modified p(AAM‐co‐EG/AAC) interpenetrating polymer network‐coated titanium implantsJournal of Biomedical Materials Research Part A, 2006
- In vitro characterization of peptide-modified p(AAm-co-EG/AAc) IPN-coated titanium implantsJournal of Orthopaedic Research, 2006