Priming mobilized peripheral blood mononuclear cells with the “activated platelet supernatant” enhances the efficacy of cell therapy for myocardial infarction of rats

Abstract

Various methods are used to augment the efficacy of cell therapy in myocardial infarction (MI). In this study, we used the 'Activated Platelet Supernatant (APS)' to prime autologous 'Granulocyte colony-stimulating factor mobilized peripheral blood mononuclear cells ((mob) PBMCs)', and investigated the efficacy of cell-based therapy in MI.Rat (mob) PBMCs were isolated after daily subcutaneous injections of G-CSF at 100 μg/kg for 3 days. APS was isolated separately after activating rat platelets with thrombin 0.5 U/ml for two hours. Priming was done with APS for 6 hours. To check the paracrine effect of primed (mob) PBMCs, we used the 36 hour culture supernatant of the primed cells. A rat MI model was used for an in vivo model.Cytokines such as IL-1β, IL-10 and TGFβ were 3.7±0.9-fold, 3.4±1.2-fold, and 1.2±0.1-fold higher in APS, respectively, compared to naïve platelet supernatant. By APS-priming, (mob) PBMCs showed M2 polarization and upregulation of angiogenic molecules (i.e. TEK, IL-10, CXCL1 and CX3CR1). APS-primed (mob) PBMCs had a 2.3-fold increased adhesion ability, induced by upregulated integrins. Rat endothelial cells cultured in the 36-hour culture supernatant of APS-primed (mob) PBMCs, showed a 1.6-fold augmented proliferation and capillary network formation. In vivo transplantation of APS-primed (mob) PBMCs into rat MI models showed a significant trend of reduction in fibrosis area (p=0.001) and wall thinning (p=0.030), which lead to improvement in cardiac function measured by echocardiography.Our data reveals that APS-priming can enhance the wound healing potential of (mob) PBMCs. APS-priming may be a promising method for cell-based therapy of MI. This article is protected by copyright. All rights reserved.