Combining genetic and biochemical approaches to identify functional molecular contact points
- 1 December 2006
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 8 (1), 77-86
- https://doi.org/10.1251/bpo121
Abstract
Protein-protein interactions are required for many viral and cellular functions and are potential targets for novel therapies. Here we detail a series of genetic and biochemical techniques used in combination to find an essential molecular contact point on the duck hepatitis B virus polymerase. These techniques include differential immunoprecipitation, mutagenesis and peptide competition. The strength of these techniques is their ability to identify contact points on intact proteins or protein complexes employing functional assays. This approach can be used to aid identification of putative binding sites on proteins and protein complexes which are resistant to characterization by other methods.Keywords
This publication has 26 references indexed in Scilit:
- Design and biological activities of novel inhibitory peptides for SARS-CoV spike protein and angiotensin-converting enzyme 2 interactionAntiviral Research, 2006
- Identification of an Essential Molecular Contact Point on the Duck Hepatitis B Virus Reverse TranscriptaseJournal of Virology, 2005
- Promises and challenges of targeting Bcl-2 anti-apoptotic proteins for cancer therapyBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2004
- Specific Residues in the Connector Loop of the Human Cytomegalovirus DNA Polymerase Accessory Protein UL44 Are Crucial for Interaction with the UL54 Catalytic SubunitJournal of Virology, 2004
- BPR0L075, a Novel Synthetic Indole Compound with Antimitotic Activity in Human Cancer Cells, Exerts Effective Antitumoral Activityin VivoCancer Research, 2004
- Inhibition of Human Cytomegalovirus DNA Polymerase by C-Terminal Peptides from the UL54 SubunitJournal of Virology, 2003
- Protein–protein interactions as targets for antiviral chemotherapyReviews in Medical Virology, 2002
- In Vitro Reconstitution of Functional Hepadnavirus Reverse Transcriptase with Cellular Chaperone ProteinsJournal of Virology, 2002
- A New Potent HIV-1 Reverse Transcriptase InhibitorOnline Journal of Public Health Informatics, 1999
- Phage DisplayChemical Reviews, 1997