Encapsulation of Berberis vulgaris Anthocyanins into Nanoliposome Composed of Rapeseed Lecithin: A Comprehensive Study on Physicochemical Characteristics and Biocompatibility
Open Access
- 25 February 2021
- Vol. 10 (3), 492
- https://doi.org/10.3390/foods10030492
Abstract
In the present study, nanoliposomes composed of rapeseed lecithin were used for the encapsulation of anthocyanin compounds (AC). The nanoliposomes were prepared using hydration and ultrasound combined method, and the effect of AC concentration (4.5, 6.75, 9% w/w) on the characteristics of nanoliposomes including particle size, polydispersity index (PDI), zeta potential, and the encapsulation efficiency (EE) of nanoliposomes with and without AC were studied. The results suggested the fabricated nanoliposomes had a size range of 141–196 nm, negative zeta potential and narrow particle size distribution. Further, the samples containing 9% extract had the maximum EE (43%). The results showed elevation of AC concentration resulted in increased particle size, PDI, EE, and surface charge of nanoparticles. The presence of AC extract led to diminished membrane fluidity through the hydrophobic interactions with the hydrocarbon chain of fatty acids. TEM images suggested that the nanoliposomes were nearly spherical and the AC caused their improved sphericity. Further, in vitro biocompatibility tests for human mesenchymal (MSC) and fibroblast (FBL) cells indicated nanoparticles were not toxic. Specifically, the best formulations with the maximum compatibility and bioavailability for MSC and FBL cells were AC-loaded nanoliposomes with concentrations of 0.5 mL/mg and 10.3 mL/µg and, respectively.This publication has 34 references indexed in Scilit:
- Elaboration and characterization of nanoliposome made of soya; rapeseed and salmon lecithins: Application to cell cultureColloids and Surfaces B: Biointerfaces, 2012
- Epigallocatechin-3-gallate (EGCG) protects against chromate-induced toxicity in vitroToxicology and Applied Pharmacology, 2012
- Optimization and characterization of liposome formulation by mixture designThe Analyst, 2011
- Mechanism of seedlessness in Iranian seedless barberry (Berberis vulgaris L. var. asperma)Scientia Horticulturae, 2010
- Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradationTrends in Food Science & Technology, 2010
- Calcein permeation across phosphatidylcholine bilayer membrane: Effects of membrane fluidity, liposome size, and immobilizationColloids and Surfaces B: Biointerfaces, 2009
- Effect of resveratrol incorporated in liposomes on proliferation and UV-B protection of cellsInternational Journal of Pharmaceutics, 2008
- A pharmacological study on Berberis vulgaris fruit extractJournal of Ethnopharmacology, 2005
- Encapsulation efficiency of water-soluble and insoluble drugs in liposomes prepared by the microencapsulation vesicle methodInternational Journal of Pharmaceutics, 2005
- Decreased oxidative stress in patients with ulcerative colitis supplemented with fish oil ω-3 fatty acidsNutrition, 2003