LINC00266-1/miR-548c-3p/SMAD2 feedback loop stimulates the development of osteosarcoma
Open Access
- 24 July 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Death & Disease
- Vol. 11 (7), 1-12
- https://doi.org/10.1038/s41419-020-02764-8
Abstract
Osteosarcoma (OS) is one of the most common primary bone malignancies and accounts for 3.4% of pediatric tumors. Its 5-year survival is as low as about 20%. Differentially expressed lncRNAs in OS profiling were searched in the downloaded profile of GSE12865. As a result, LINC00266-1 was detected to be upregulated in both GSE12865 and OS tissues we collected. SMAD2 was the downstream target binding to promoter sites of LINC00266-1, displaying a positive regulatory interaction. Knockdown of LINC00266-1 suppressed the proliferative and metastatic abilities, and promoted the apoptosis in OS cells. Besides, knockdown of LINC00266-1 significantly alleviated the growth of OS in vivo. MiR-548c-3p was the sponge miRNA of LINC00266-1, which was able to reverse the regulatory effects of LINC00266-1 on OS cell phenotypes. Moreover, miR-548c-3p bound to the 3′-UTR of SMAD2 and thus downregulated SMAD2. Overexpression of SMAD2 partially reversed the regulatory effects of LINC00266-1 on OS cell phenotypes. Finally, we have identified that LINC00266-1/miR-548c-3p/SMAD2 feedback loop was responsible for stimulating the development of OS.Keywords
This publication has 35 references indexed in Scilit:
- LncRNA MALAT1 Promotes Cancer Metastasis in Osteosarcoma via Activation of the PI3K-Akt Signaling PathwayCellular Physiology and Biochemistry, 2018
- Long non-coding RNA SNHG5 sponges miR-26a to promote the tumorigenesis of osteosarcoma by targeting ROCK1Biomedicine & Pharmacotherapy, 2018
- Long noncoding RNA HOXD-AS1 aggravates osteosarcoma carcinogenesis through epigenetically inhibiting p57 via EZH2Biomedicine & Pharmacotherapy, 2018
- AFAP1‐AS1: A novel oncogenic long non‐coding RNA in human cancersCell Proliferation, 2017
- A meta-analysis of randomized control trials of surgical methods with osteosarcoma outcomesJournal of Orthopaedic Surgery and Research, 2017
- Overexpression of long non-coding RNA MFI2 promotes cell proliferation and suppresses apoptosis in human osteosarcomaOncology Reports, 2016
- Different MicroRNA Expression Levels in Gastric Cancer Depending on Helicobacter pylori InfectionGut and Liver, 2015
- MicroRNA Expression Profile of Primary Prostate Cancer Stem Cells as a Source of Biomarkers and Therapeutic TargetsEuropean Urology, 2015
- lncRNAdb v2.0: expanding the reference database for functional long noncoding RNAsNucleic Acids Research, 2014
- Long Noncoding RNAs: Cellular Address Codes in Development and DiseaseCell, 2013