ŞEBİNKARAHİSAR CEVİZ ÇEŞİDİNDE AFLATOKSİN İÇERİĞİNİN ELISA YÖNTEMİYLE BELİRLENMESİ

Abstract

Aflatoxins are carcinogenic toxic metabolites for human foods and animal feeds in terms of pharmaceutical produced by Aspergillus flavus and Aspergillus parasiticus. This study was carried out to determine the aflatoxin changes in Şebinkarahisar walnut under different seasonal changes between the years by enzyme-linked immunosorbent assay (ELISA) method. The presence of aflatoxin was found in all of the samples within measurable limits. But in none of the total 20 different walnut samples examined, aflatoxin was not observed above the limits determined by the Turkish Food Codex Contaminants Regulation. Each treatment included 3 wells were carried out triplicates to determination of AFT and AFB1 and correlation analysis showed a significant relationship between AFB1 and AFT for 2 different years. In addition, although there was an increase in aflatoxin content due to seasonal changes between the years, there was no statistically significant difference and it was supported by correlation analysis.