Using Terpene Synthase Plasticity in Catalysis: On the Enzymatic Conversion of Synthetic Farnesyl Diphosphate Analogues
Open Access
- 14 September 2021
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 27 (63), 15644-15649
- https://doi.org/10.1002/chem.202103049
Abstract
Four synthetic farnesyl diphosphate analogues were enzymatically converted with three bacterial sesquiterpene synthases, including β-himachalene synthase (HcS) and ( Z )-γ-bisabolene synthase (BbS) from Cryptosporangium arvum , and germacrene A synthase (SmTS6) from Streptomyces mobaraensis . These enzyme reactions not only yielded several previously unknown compounds, showing that this approach opened the door to a new chemical space, but substrates with blocked or altered reactivities also gave interesting insights into the cyclisation mechanisms and the potential to catalyse reactions with different initial cyclisation modes.Funding Information
- Deutsche Forschungsgemeinschaft (DI1536/7-2)
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