New Search

Export article

Animal Model of Non-Alcoholic Steatohepatitis in Histology Department Sohag University

, Doha S. Mohammed, Nesreen G. Abd El-Haliem, Ahmed O. Ahmed, Eman E. Abu-Dief

Abstract: Non-alcoholic fatty liver disease (NAFLD) is characterized by the deposition of triglycerides in the hepatocytes causing their injury resulting in non-alcoholic steatohepatitis (NASH), fibrosis, and cirrhosis, as a healing response, and may progress to hepatocellular carcinoma (HCC). We aimed to establish an animal model for NAFLD/NASH by the use of a high-fat diet. A total number of 30 adult male mice were categorized into two groups; control, high-fat diet (HFD) 15 animals each. The livers of these animals were excised and processed for histological examination for the progress of NAFLD/NASH changes. At the end of the experiment, the mice were slaughtered at 11, 13, 15, 17-week intervals. Liver specimens and blood samples from all animal groups were collected. The liver specimens were processed and stained with hematoxylin & eosin. Blood samples were processed for liver functions. Our results demonstrate that NASH was established 17 weeks after high fat diet administration
Keywords: hepatocytes / Model / alcoholic / adult / animal / steatohepatitis / functions / liver / NAFLD/NASH

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

Share this article

Click here to see the statistics on "Sohag Medical Journal" .
Back to Top Top