Research tools, such as model organisms, cell lines, and antibodies, are essential to designing and executing successful biological experiments. These resources are often shared or made commercially available to support scientific progress. Given the fast pace of research, it can be difficult to keep track of the large number of available tools. Moreover, for those new to a particular disease area, learning about the array of tools available can be a major impediment. Our experience in the neurofibromatosis field has shown that researchers struggle to identify the research tools available to them, determine where tools can be acquired, and understand what tools are most well-suited for which experiments. While a variety of databases exist to help researchers find useful research tools, these databases often a) are specific to one type of research tool while being disease-agnostic, b) provide only high-level information, c) do not contain information about in-development models, and d) do not contain observational data for the research tools. To address these limitations, we created the Neurofibromatosis Research Tools Database, a user-friendly, open-access database and companion portal designed to help the neurofibromatosis type 1 (NF1) research community easily find, obtain, and use NF1-relevant research tools. This prototype database catalogs a wide variety of NF1-relevant research tools using databases such as Cellosaurus, AntibodyRegistry, RRID Portal, among others, as well as information provided in literature and from the NF community. We aggregated and curated metadata for NF1-relevant animal models, cell lines, genetic reagents, antibodies, and biobanks. The database includes core metadata for all tools, e.g., name, type of tool, synonyms, developer, as well as tool type-specific metadata, e.g., for cell lines or animal models, the type of cancer that the model recapitulates. The database is also designed to store observational data contributed directly from the research community. Our companion web portal allows users to search and filter this database interactively and easily explore these tools. This website was built within the NF Data Portal (nf.synapse.org), and is available at tools.nf.synapse.org. Community members can actively contribute to the growth of the database and portal by submitting information about the reliability, biology, usage, and other observations on each research tool. By collating and curating this information and surfacing it in an open-access exploration portal, we anticipate that this database will serve as a valuable resource to help the NF community discover, understand, and use NF1 research tools. Citation Format: Brynn Zalmanek, James Goss, Mialy DeFelice, Jay Hodgson, Ashley Clayton, Stockard Simon, Marco Marasca, Julie Bletz, James A. Eddy, Milen Nikolov, Kevin Boske, Ljubomir Bradic, Jineta Banerjee, Kalyan Vinnakota, Caroline Morin, YooRi Kim, Robert J. Allaway. NF Research Tools Database: A knowledge base of experimental research tools for neurofibromatosis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 1675.