Bir Deniz Akvaryumunda Salpa Balıklarında (Sarpa salpa) Vibrio harveyi Enfeksiyonu Olgusu: ilk rapor
Open Access
- 1 September 2018
- journal article
- Published by Suleyman Demirel Universitesi Egirdir Su Urunleri Fakultesi Dergisi in Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi
- Vol. 14 (3), 166-172
- https://doi.org/10.22392/egirdir.371581
Abstract
Tr en Bu çalışma, bir deniz akvaryumundan alınan hasta salpa balıklarında (Sarpa salpa) hastalığa neden olan etken veya etkenleri tanımlamak üzere yapılmıştır. Hasta balıkların vücudunda hafif şiddette deri renginde açılma ile birlikte pul kayıpları gözlenmiştir. İç bakıda, karaciğer ve bağırsak dokularının hiperemik ve kas dokusunun da hemorajik olduğu tespit edilmiştir. Doku kesitleri histopatolojik olarak incelendiğinde, enfeksiyonun; özellikle solungaç epitelyumu, bağırsak mukozası, kalp kası ve dalağın hematopoetik dokusunu etkilediği belirlenmiştir. Fenotipik ve biyokimyasal özellikleri ve SSU rRNA dizileme sonuçlarına göre; incelenen tüm iç organlardan (böbrek, dalak, karaciğer) ve kan dokusundan Vibrio harveyi bakterisi izole edilmiştir. Buna ek olarak, incelenen balıklardan sadece birinin kan dokusundan ve dalağından alınan örneklerde Vibrio vulnificus ve Pseudoalteromonas piscicida bakterileri de izole edilmiştir. In this study, moribund salema (Sarpa salpa) individuals from a public aquarium were investigated to identify the cause of disease. A minor scale loss and partial skin depigmentation were observed in the diseased fish. Internal investigation of fish revealed hyperemia in the liver and the intestines and haemorrhages in the muscle tissues. When the histopathological sections of tissues were examined, it was determined that the bacterial infection affected especially the gill epithelium, the intestinal mucosa, the heart muscle and hemopoietic tissues of the spleen. According to phenotypic and biochemical characteristics as well as SSU rRNA sequencing results, the isolated bacteria was Vibrio harveyi and consistently seen all the examined samples of visceral organs including kidney, spleen, liver and blood. However, we need to emphasize that Vibrio vulnificus and Pseudoalteromonas piscicida bacteria were also isolated from blood and spleen tissues of one sick fish among others.Keywords
This publication has 15 references indexed in Scilit:
- Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneataDiseases of Aquatic Organisms, 2009
- Clustal W and Clustal X version 2.0Bioinformatics, 2007
- Vibrio harveyi: a significant pathogen of marine vertebrates and invertebratesLetters in Applied Microbiology, 2006
- Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious DiseasesClinical Microbiology Reviews, 2004
- Virulence and Molecular Typing of Vibrio harveyi Strains Isolated from Cultured Dentex, Gilthead Sea Bream and European Sea BassSystematic and Applied Microbiology, 2003
- Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular productsJournal of Fish Diseases, 2003
- A Greedy Algorithm for Aligning DNA SequencesJournal of Computational Biology, 2000
- Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L.Journal of Fish Diseases, 1999
- Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996Aquaculture, 1998
- Association of Vibrio harveyi with mortalities in cultured marine fish in KuwaitAquaculture, 1995