Loliolide, a New Therapeutic Option for Neurological Diseases? In Vitro Neuroprotective and Anti-Inflammatory Activities of a Monoterpenoid Lactone Isolated from Codium tomentosum
Open Access
- 14 February 2021
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 22 (4), 1888
- https://doi.org/10.3390/ijms22041888
Abstract
Parkinsons Disease (PD) is the second most common neurodegenerative disease worldwide, and is characterized by a progressive degeneration of dopaminergic neurons. Without an effective treatment, it is crucial to find new therapeutic options to fight the neurodegenerative process, which may arise from marine resources. Accordingly, the goal of the present work was to evaluate the ability of the monoterpenoid lactone Loliolide, isolated from the green seaweed Codium tomentosum, to prevent neurological cell death mediated by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y cells and their anti-inflammatory effects in RAW 264.7 macrophages. Loliolide was obtained from the diethyl ether extract, purified through column chromatography and identified by NMR spectroscopy. The neuroprotective effects were evaluated by the MTT method. Cells’ exposure to 6-OHDA in the presence of Loliolide led to an increase of cells’ viability in 40%, and this effect was mediated by mitochondrial protection, reduction of oxidative stress condition and apoptosis, and inhibition of the NF-kB pathway. Additionally, Loliolide also suppressed nitric oxide production and inhibited the production of TNF-α and IL-6 pro-inflammatory cytokines. The results suggest that Loliolide can inspire the development of new neuroprotective therapeutic agents and thus, more detailed studies should be considered to validate its pharmacological potential.Funding Information
- Fundação para a Ciência e a Tecnologia (UID/04292/2020, UIDP/04046/2020, UIDB/04046/2020, PTDC/BIA-OUT/29250/2017, PTDC/ BIA-BQM/28355/2017, SFRH/BD/103255/2014)
- European Regional Development Fund (PTDC/ BIA-BQM/28355/2017, Centro-01-0145-FEDER-000018)
- Programa Operacional Regional do Centro (POCI-01-0145-FEDER-029250)
This publication has 72 references indexed in Scilit:
- Rutin, a bioflavonoid antioxidant protects rat pheochromocytoma (PC-12) cells against 6-hydroxydopamine (6-OHDA)-induced neurotoxicityInternational Journal of Molecular Medicine, 2013
- A Spirulina-Enhanced Diet Provides Neuroprotection in an α-Synuclein Model of Parkinson's DiseasePLOS ONE, 2012
- Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune DiseaseCritical Reviews™ in Immunology, 2012
- Neuroinflammation in Parkinson's disease: Its role in neuronal death and implications for therapeutic interventionNeurobiology of Disease, 2010
- Mechanisms Underlying Inflammation in NeurodegenerationCell, 2010
- The NF- B Family of Transcription Factors and Its RegulationCold Spring Harbor Perspectives in Biology, 2009
- Role of reactive oxygen species in the neurotoxicity of environmental agents implicated in Parkinson's diseaseFree Radical Biology & Medicine, 2008
- Selective inhibition of NF-κB activation prevents dopaminergic neuronal loss in a mouse model of Parkinson's diseaseProceedings of the National Academy of Sciences of the United States of America, 2007
- Marine algae that display anti-tumorigenic activity againstAgrobacterium tumefaciensFEMS Microbiology Letters, 1995
- Sterols from marine green alga Codium decorticatumPhytochemistry, 1993