Long-term and in vivo assessment of Aβ protein-induced brain atrophy in a zebrafish model by optical coherence tomography

Abstract

In this study, a neurotoxicity model of zebrafish induced by amyloid beta (A beta) protein was developed and evaluated in vivo by optical coherence tomography (OCT). A beta protein and phosphate buffer saline (PBS) were separately injected into the head of two groups of adult zebrafish (n = 6 per group). Congo-red staining results confirmed that A beta protein had penetrated into brain tissue. All zebrafish were imaged with OCT on the 0th, 5th, 10th, 15th and 20th day postinjection. OCT images showed that PBS is not toxic to brain tissue. However, significant brain atrophy could be seen in the OCT images of zebrafish injected with A beta-protein that was verified by histological consequences. In addition, zebrafish in the model group showed memory decline in behavioral tests. This study verified the feasibility of in vivo long-term assessment of A beta protein-induced brain atrophy in adult zebrafish by OCT that has great potential to be applied in the neurological diseases research.