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Partridge and embryonated partridge egg as new preclinical models for candidiasis

, , Iraj Sharifi, Zohreh Salari, Ehsan Salarkia, Reza Kheirandish, Kazem Dehghantalebi, Maziar Jajarmi, Seyedeh Saedeh Mosallanejad, Shahriar Dabiri, Alireza Keyhani
Published: 22 January 2021
Scientific Reports , Volume 11, pp 1-16; doi:10.1038/s41598-021-81592-y

Abstract: Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge and embryonated partridge egg. First, we tested the induction of systemic candidiasis in partridge and embryonated partridge egg. Finally, interaction between virulence factors of C. albicans and Bcl-2 family members was predicted. We observed that embryonic infection causes a decrease in survival time and at later embryonic days (11–12th), embryos showed lesions. Morphometric analysis of the extra-embryonic membrane (EEM) vasculature showed that vascular apoptotic effect of C. albicans was revealed by a significant reduction in capillary area. In immunohistochemistry assay, low expression of Bcl-2 and increased expression of Bax confirmed apoptosis. The gene expression of Bax and Bcl-2 was also altered in fungi-exposed EEM. Ourin silico simulation has shown an accurate interaction between aspartic proteinase, polyamine oxidase, Bcl-2 and BAX. We observed that the disease was associated with adverse consequences, which were similar to human candidiasis. Acquired results support the idea that partridge and embryonated partridge egg can be utilized as appropriate preclinical models to investigate the pathological effects of candidiasis.
Keywords: fungi / fungal pathogenesis

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