Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması
- 1 January 2021
- journal article
- research article
- Published by Kafkas University in Kafkas Universitesi Veteriner Fakultesi Dergisi
- Vol. 27 (2), 225-233
- https://doi.org/10.9775/kvfd.2020.25106
Abstract
Although the clinical and pathological findings are important in the diagnosis of listeriosis, to isolation or to be shown the presence of the bacterium must be required for the definitive diagnosis. This study aims to investigate the availability of imprint cytological (IC) and immunocytochemical (ICC) methods in comparison with histopathological and immunohistochemical (IHC) methods for the rapid diagnosis of encephalitic listeriosis. In the study, the touching and smear preparations taken from the pons and medulla oblongata of 25 ruminants suspected with listeriosis by neurological symptoms were stained with modified giemsa and also with ICC technic for revealing antigens, as a new method. Same tissue sections were stained with Hematoxylin&Eosin and IHC methods too, and examined under light microscope by scoring. In IC examinations, there were intensive neutrophils in 14 cases and few neutrophils in 4 cases, and no neutrophils were observed in 7 cases. In histopathological examinations, 13 of these 14 cases revealed typical microabscesses and listeria positivity in IHC staining. ICC positivity was detected in 12 (92.3%) of the listeria positive 13 cases. A highly positive correlation was observed among cytology (14), ICC (12), histopathology and IHC (13) scores (r2> 0.8; P<0.01). In conclusion, the cytological examination of the pons and medulla oblongata of listeriosis-suspected ruminants revealed that a rapid pre- diagnosis could be made with the presence of intense neutrophils. Also, with ICC staining of cytological preparations, the diagnosis could be performed with 92.3% accuracy. Since ICC is an easy and fast method, it is concluded that it can be used safely especially in field studies, along with cytological examination.This publication has 13 references indexed in Scilit:
- Changes on the activity of cholinesterase's in an immunomodulatory response of cattle infected by Listeria monocytogenesMicrobial Pathogenesis, 2018
- Listeria monocytogenes: towards a complete picture of its physiology and pathogenesisNature Reviews Microbiology, 2017
- Outbreak investigation identifies a single Listeria monocytogenes strain in sheep with different clinical manifestations, soil and waterVeterinary Microbiology, 2015
- Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive reviewVeterinary Quarterly, 2015
- Listeria monocytogenes Spreads within the Brain by Actin-Based Intra-Axonal MigrationInfection and Immunity, 2015
- Use of Immunoperoxidase Technique in Smears Prepared from Vaginal Secretions in Early Diagnosis of Listerial Abortions in CattleKafkas Universitesi Veteriner Fakultesi Dergisi, 2013
- Listeria monocytogenes Infection of the Alimentary Tract (Enteric Listeriosis) of Sheep in New ZealandJournal of Comparative Pathology, 2012
- Neuropathogenesis of Naturally Occurring Encephalitis Caused by Listeria monocytogenes in RuminantsBrain Pathology, 2010
- Neuropathological survey of fallen stock: Active surveillance reveals high prevalence of encephalitic listeriosis in small ruminantsVeterinary Microbiology, 2008
- Pathological and epidemiological investigations into listerial encephalitis in sheepSmall Ruminant Research, 2007