Evaluation of Novel Virulent Phages Infecting the Aeromonas Hydrophila and Escherichia coli Isolated from Sewage Water Samples
- 18 July 2020
- journal article
- research article
- Published by Egypts Presidential Specialized Council for Education and Scientific Research in Egyptian Journal of Botany
- Vol. 60 (3), 805-823
- https://doi.org/10.21608/ejbo.2020.20950.1411
Abstract
Multidrug-resistant bacteria are now emerging for almost all the present-day antibiotics. Aeromonas hydrophila D2007 and Escherichia coli W102 were isolated from fresh food and drinking water samples and they were resistant to 57.14% and 85.71% of tested common antibiotics respectively. Three bacteriophages (phages) were isolated from sewage samples. Morphological examinations suggested that phage ΦAHP7, which infects A. hydrophila D2007, belongs to the Myoviridae family and other phages ΦECP8 and ΦECP9 capable of lysing E. coil W102 belongs to Siphoviridae and Podoviridae families respectively. For the three phages, the optimal multiplicity of infection (MOI) was calculated to be 0.001. Phages were characterized by determining their host range and stability in pHs, temperatures, and salinity. The latent periods of phages ΦAHP7, ΦECP8, and ΦECP9 were 10, 20 and 10 min with average burst sizes of 53.5 ±0.5, 26.5 ±0.5 and 67.5 ±0.5 phages per infected cell respectively. The three phages gradually reduced OD600 and are able to stop the growth of A. hydrophila D2007 and E. coli W102 in vitro at a low MOI of 0.001. Phages ΦAHP7, ΦECP8, and ΦECP9 treatments achieved 1.55, 1.68 and 2.28 log CFU/g (p <0.01) reduction of viable bacterial number in red cabbage and 1.48, 1.38 and 1.68 log CFU/g (p <0.01) reduction in tomato after 30 min at room temperature (28°C) respectively. Applications of lytic ΦAHP7, ΦECP8, and ΦECP9 bacteriophages lead to a rapid reduction of A. hydrophila D2007 and E. coli W102 counts in fresh food for human consumption.Keywords
This publication has 52 references indexed in Scilit:
- T4-Like Phage Bp7, a Potential Antimicrobial Agent for Controlling Drug-Resistant Escherichia coli in ChickensApplied and Environmental Microbiology, 2013
- Bacteriophages with Potential for Inactivation of Fish Pathogenic Bacteria: Survival, Host Specificity and Effect on Bacterial Community StructureMarine Drugs, 2011
- The influence of external factors on bacteriophages—reviewFolia Microbiologica, 2011
- Mycoplasma hyorhinisinfection in gastric carcinoma and its effects on the malignant phenotypes of gastric cancer cellsBMC Gastroenterology, 2010
- Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminate Escherichia coli O157:H7 from lettuce leavesInternational Journal of Food Microbiology, 2009
- Bacteriophages Reduce Experimental Contamination of Hard Surfaces, Tomato, Spinach, Broccoli, and Ground Beef by Escherichia coli O157:H7Applied and Environmental Microbiology, 2008
- Phage inactivation of foodborne pathogens on cooked and raw meatFood Microbiology, 2008
- The importance of Aeromonas hydrophila in food safetyFood Control, 2006
- Therapeutic use of phage cocktail for controlling Escherichia coli O157:H7 in gastrointestinal tract of miceJournal of Bioscience and Bioengineering, 2005
- Bacteriophage Ecology in Commercial Sauerkraut FermentationsApplied and Environmental Microbiology, 2003