Magnetic bead-enzyme assemble for triple-parameter telomerase detection at single-cell level
- 1 September 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 412 (22), 5283-5289
- https://doi.org/10.1007/s00216-020-02741-2
Abstract
In this work, we developed a triple-parameter strategy for the detection of telomerase activity from cancer cells and urine samples. This strategy was developed based on magnetic bead-enzyme hybrids combined with fluorescence analysis, colorimetric assay, or adenosine triphosphate (ATP) meter as readout. The application of magnetic bead-enzyme hybrids has the advantages of magnetic separation and signal amplification. These detection methods can be used individually or in combination to achieve the optimal sensing performance and make the results more convincing. Among them, the ATP meter with portable size had easy operation and low cost, and this response strategy provided a higher sensitivity at the single-cell level. The designed strategy was suitable as naked-eye sensor and point-of-care testing (POCT) for rapid assaying of telomerase activity.Funding Information
- National Natural Science Foundation of China (81772737)
- Natural Science Foundation of Guangdong Province (2018B030306046)
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