A dynamic mouse peptidome landscape reveals probiotic modulation of the gut-brain axis
- 28 July 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Signaling
- Vol. 13 (642)
- https://doi.org/10.1126/scisignal.abb0443
Abstract
Certain probiotics have beneficial effects on the function of the central nervous system through modulation of the gut-brain axis. Here, we describe a dynamic landscape of the peptidome across multiple brain regions, modulated by oral administration of different probiotic species over various times. The spatiotemporal and strain-specific changes of the brain peptidome correlated with the composition of the gut microbiome. The hippocampus exhibited the most sensitive response to probiotic treatment. The administration of heat-killed probiotics altered the hippocampus peptidome but did not substantially change the gut microbiome. We developed a literature-mining algorithm to link the neuropeptides altered by probiotics with potential functional roles. We validated the probiotic-regulated role of corticotropin-releasing hormone by monitoring the hypothalamic-pituitary-adrenal axis, the prenatal stress–induced hyperactivity of which was attenuated by probiotics treatment. Our findings provide evidence for modulation of the brain peptidome by probiotics and provide a resource for further studies of the gut-brain axis and probiotic therapies.Keywords
Funding Information
- National Natural Science Foundation of China (21675006)
- Ministry of Science and Technology of the People’s Republic of China (2017YFA0505702)
- Ministry of Science and Technology of the People’s Republic of China (2016YFA0501302)
- Key Research Program of China (BWS17J025)
- NS Bio Japan (20170403)
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