Insights into the Gryllus bimaculatus Immune-Related Transcriptomic Profiling to Combat Naturally Invading Pathogens
Open Access
- 18 October 2020
- journal article
- research article
- Published by MDPI AG in Journal of Fungi
- Vol. 6 (4), 232
- https://doi.org/10.3390/jof6040232
Abstract
Natural pathogen pressure is an important factor that shapes the host immune defense mechanism. The current study primarily aimed to explore the molecular basis of the natural immune defense mechanism of a sporadic pest, Gryllus bimaculatus, during swarming by constructing cDNA libraries of the female mid-gut, male mid-gut, testes, and ovaries. The Illumina HiSeq platform generated an average of 7.9 G, 11.77 G, 10.07 G, and 10.07 G bases of outputs from the male mid-gut, female mid-gut, testes, and ovaries and libraries, respectively. The transcriptome of two-spotted field crickets was assembled into 233,172 UniGenes, which yielded approximately 163.58 million reads. On the other hand, there were 43,055 genes in common that were shared among all the biological samples. Gene Ontology analysis successfully annotated 492 immune-related genes, which comprised mainly Pattern Recognition Receptors (62 genes), Signal modulators (57 genes), Signal transduction (214 genes), Effectors (36 genes), and another immune-related 123 genes. In summary, the identified wide range of immune-related genes from G. bimaculatus indicates the existence of a sophisticated and specialized broad spectrum immune mechanism against invading pathogens, which provides, for the first time, insights into the molecular mechanism of disease resistance among two-spotted field crickets.Keywords
This publication has 72 references indexed in Scilit:
- The Ras protein superfamily: Evolutionary tree and role of conserved amino acidsThe Journal of cell biology, 2012
- RNA-seq: technical variability and samplingBMC Genomics, 2011
- Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a β-1,3-glucanase-related protein in Manduca sexta larval plasmaInsect Biochemistry and Molecular Biology, 2011
- Immunity and other defenses in pea aphids, Acyrthosiphon pisumGenome Biology, 2010
- A Serpin that Regulates Immune Melanization in the Respiratory System of DrosophilaDevelopmental Cell, 2008
- The Antibacterial Protein Lysozyme Identified as the Termite Egg Recognition PheromonePLOS ONE, 2007
- Deficiency in the LIM-only protein Fhl2 impairs skin wound healingThe Journal of cell biology, 2007
- Lentinula edodes tlg1 Encodes a Thaumatin-Like Protein That Is Involved in Lentinan Degradation and Fruiting Body SenescencePlant Physiology, 2006
- Pathogen Recognition and Innate ImmunityCell, 2006
- KEGG: Kyoto Encyclopedia of Genes and GenomesNucleic Acids Research, 2000