Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration
Open Access
- 29 June 2020
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (13), 4661
- https://doi.org/10.3390/ijms21134661
Abstract
Bone remodeling and repair require osteogenic cells to reach the sites that need to be rebuilt, indicating that stimulation of osteoblast migration could be a promising osteoanabolic strategy. We showed that purified δ-tocotrienol (δ-TT, 10 μg/mL), isolated from commercial palm oil (Elaeis guineensis) fraction, stimulates the migration of both MC3T3-E1 osteoblast-like cells and primary human bone marrow mesenchymal stem cells (BMSC) as detected by wound healing assay or Boyden chamber assay respectively. The ability of δ-TT to promote MC3T3-E1 cells migration is dependent on Akt phosphorylation detected by Western blotting and involves Wnt/β-catenin signalling pathway activation. In fact, δ-TT increased β-catenin transcriptional activity, measured using a Nano luciferase assay and pretreatment with procaine (2 µM), an inhibitor of the Wnt/β-catenin signalling pathway, reducing the wound healing activity of δ-TT on MC3T3-E1 cells. Moreover, δ-TT treatment increased the expression of β-catenin specific target genes, such as Osteocalcin and Bone Morphogenetic Protein-2, involved in osteoblast differentiation and migration, and increased alkaline phosphatase and collagen content, osteoblast differentiation markers. The ability of δ-TT to enhance the recruitment of BMSC, and to promote MC3T3-E1 differentiation and migratory behavior, indicates that δ-TT could be considered a promising natural anabolic compound.Keywords
Funding Information
- Fondazione Cariplo (0834/2015)
This publication has 56 references indexed in Scilit:
- Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient RatsInternational Journal of Endocrinology, 2012
- Effects of Palm Vitamin E on Bone-Formation-Related Gene Expression in Nicotine-Treated RatsEvidence-Based Complementary and Alternative Medicine, 2012
- A specific role for phosphoinositide 3-kinase and AKT in osteoblasts?Frontiers in Endocrinology, 2012
- Effects of Tocotrienol and Lovastatin Combination on Osteoblast and Osteoclast Activity in Estrogen-Deficient OsteoporosisEvidence-Based Complementary and Alternative Medicine, 2012
- Mechano-transduction in Osteoblastic Cells Involves Strain-regulated Estrogen Receptor α-mediated Control of Insulin-like Growth Factor (IGF) I Receptor Sensitivity to Ambient IGF, Leading to Phosphatidylinositol 3-Kinase/AKT-dependent Wnt/LRP5 Receptor-independent Activation of β-Catenin SignalingPublished by Elsevier BV ,2010
- Bone Physiology, Disease and TreatmentClinical Pharmacokinetics, 2010
- Phosphorylation of β-Catenin by AKT Promotes β-Catenin Transcriptional ActivityPublished by Elsevier BV ,2007
- Bone morphogenetic protein-2 promotes the haptotactic migration of murine osteoblastic and osteosarcoma cells by enhancing incorporation of integrin β1 into lipid raftsExperimental Cell Research, 2006
- Inhibition of Histone Acetylation as a Tool in Bone Tissue EngineeringTissue Engineering, 2006
- Glucocorticoids Inhibit the Transcriptional Activity of LEF/TCF in Differentiating Osteoblasts in a Glycogen Synthase Kinase-3β-dependent and -independent MannerPublished by Elsevier BV ,2005