Indikator mutu pelayanan dinilai dari perilaku tenaga kesehatan khususnya perawat dalam menjaga keselamatan dirinya. Budaya organisasi, komunikasi antar perawat, pengendalian, dan hubungan tim serta pelatihan yang kurang dapat memengaruhi perilaku perawat dalam menjaga keselamatan diri. Jika keselamatan dan kesehatan perawat tidak diperhatikan akan terjadi peningkatan absensi, ketidakpuasan bekerja, produktifitas dan konsentrasi perawat dalam bekerja menurun. Penelitian bertujuan mengidentifikasi determinan perilaku perawat dalam menjaga keselamatan dirimenggunakan metode analitik korelatif pada 105 perawat pelaksana di rumah sakit dengan metode accidental sampling.Alat ukur menggunakan kuesioner dan lembar observasi. Data dianalisis menggunakan independent t test dan uji chi-square. Faktor-faktor yang mempengaruhi perilaku perawat dalam menjaga keselamatan diri:budaya organisasi (p= 0,004; CI = 1,831-38,404), komunikasi (p=0,003; CI = 1,776-14,845), pengendalian (p=0,012; CI = 1,457-14,790), hubungan tim (p=0,016; CI= 1,337- 9,035), serta pelatihan (p=0,014; CI =1,365 – 8,924). Hasil ini dapat menjadi dasar bagi rumah sakit untuk meningkatkan perilaku perawat menjaga keselamatan diri. Abstract Improving Nurses Safety through Organization Culture, Communication, Controlling Function, Team Relationships and Training. Indicators of service quality assessed from the behavior of health personnels in maintaining their safety. Organizational culture, communication between nurses, controlling function, team relationshipst hat are less and less training can influence the behavior of nurses in maintaining personal safety. If the safety and health of nurses are not considered to be an increase in attendance, unsatisfactory work, productivity and concentration of nurses in work declined. This study aimed to identify the determinants of nursing behavior in maintaining personal safety by using correlative analytic methods to 105 nurses at hospital with accidental sampling method. Data collected from questionnaires and observation sheets were analyzed using independent t test and chi-square test. Factors that influenced nurses’ behavior in maintaining personal safety were organizational culture (p = 0,004 ; CI = 1,831-38,404), communication (p= 0,003 ; CI = 1,776-14,845), controlling function (p= 0,012 ; CI = 1,457-14,790), team relationships ( p= 0,016 ; CI = 1,337-9,035), and training (p= 0,014 ; CI = 1,365-8,924). These results can be the basis for hospitals to improve the behavior of the nurses keep personal safety. Keywords: behavior, determinants, nurse, safety.