Prevalence, antibiotic susceptibility and characterization of Vibrio parahaemolyticus isolates in China
- 1 August 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 367 (16)
- https://doi.org/10.1093/femsle/fnaa136
Abstract
Vibrio parahaemolyticus is a marine and estuarine bacterium that poses a major threat to human health worldwide. In this study, from 2017 to 2019, we evaluated 900 food samples collected from China in 2017, with an aimed to determine the incidence and features of V.parahaemolyticus in ready-to-eat (RTE) foods, shrimp and fish in China. The contamination rates in these were 3.67%, 19.33%, and 10.67%, respectively. and the prevalence of V.parahaemolyticus was higher in summer than in winter. In addition, 101 V.parahaemolyticus strains were isolated. Our results suggested that most of the isolates were resistant to aminoglycosides base on the antimicrobial resistance patterns of these aquatic product isolates against 14 antimicrobial agents. Furthermore, most of the isolates were multidrug-resistant. Serotyping showed that the isolates of the O2 serotype comprised the maximum proportion. ERIC-PCR results indicated that the isolates (n = 101) could be classified into 12 clusters. There were 82 STs suggesting genetic variation and relatedness among these isolates. Our findings demonstrated the presence of V.parahaemolyticus in foods from Chinese retail markets, and show that this methodology can be used for microbiological risk assessment in China.Keywords
Funding Information
- Foshan Technology Project (1920001001759, K683820012112)
- Teaching Project (YNYJZW2019QN08)
This publication has 34 references indexed in Scilit:
- Phenotypic and Genotypic Characterization of Canadian Clinical Isolates of Vibrio parahaemolyticus Collected from 2000 to 2009Journal of Clinical Microbiology, 2014
- Prevalence and antimicrobial susceptibility of Vibrio species related to food safety isolated from shrimp cultured at inland ponds in ThailandFood Control, 2014
- MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0Molecular Biology and Evolution, 2013
- Development of O-serogroup specific PCR assay for detection and identification of Vibrio parahaemolyticusInternational Journal of Food Microbiology, 2012
- Seasonal prevalence of Vibrio species in retail shrimps with an emphasis on Vibrio parahaemolyticusFood Control, 2012
- Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistanceClinical Microbiology & Infection, 2012
- Distribution, serological and molecular characterization of Vibrio parahaemolyticus from shellfish in the eastern coast of ChinaFood Control, 2011
- Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and watersInternational Journal of Food Microbiology, 2008
- Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern ChinaInternational Journal of Food Microbiology, 2008
- Characterization of United States outbreak isolates ofVibrio parahaemolyticususing enterobacterial repetitive intergenic consensus (ERIC) PCR and development of a rapid PCR method for detection of O3:K6 isolatesFEMS Microbiology Letters, 2002