A bioinformatics workflow for the evaluation of RT-qPCR primer specificity: Application for the assessment of gene expression data reliability in toxicological studies
Open Access
- 1 March 2020
- journal article
- research article
- Published by Elsevier BV in Regulatory Toxicology and Pharmacology
- Vol. 111, 104575
- https://doi.org/10.1016/j.yrtph.2020.104575
Abstract
No abstract availableFunding Information
- Government of Canada, Canada
- Government of Canada, Canada
This publication has 53 references indexed in Scilit:
- Primer3—new capabilities and interfacesNucleic Acids Research, 2012
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reactionBMC Bioinformatics, 2012
- Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrimeNucleic Acids Research, 2012
- Identification of gene-oriented exon orthology between human and mouseBMC Genomics, 2012
- PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 updateNucleic Acids Research, 2011
- Functional Implications of Structural Predictions for Alternative Splice Proteins Expressed in Her2/neu–Induced Breast CancersJournal of Proteome Research, 2011
- Tissue Distribution and Gender-Divergent Expression of 78 Cytochrome P450 mRNAs in MiceToxicological Sciences, 2011
- GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCRDatabase: The Journal of Biological Databases and Curation, 2011
- VEGF-A splicing: the key to anti-angiogenic therapeutics?Nature Reviews Cancer, 2008
- Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalizationLaboratory Investigation, 2005