Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange
Open Access
- 29 September 2020
- Vol. 12 (10), 1101
- https://doi.org/10.3390/v12101101
Abstract
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.Keywords
Funding Information
- European Commission (2015-FED-187)
This publication has 51 references indexed in Scilit:
- Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirusVirus Genes, 2018
- Complete genome sequence of yam chlorotic necrosis virus, a novel macluravirus infecting yamArchiv für die gesamte Virusforschung, 2018
- Complete genome sequence of a new member of the genus Badnavirus, Dioscorea bacilliform RT virus 3, reveals the first evidence of recombination in yam badnavirusesArchiv für die gesamte Virusforschung, 2018
- Characterization of badnaviruses infecting Dioscorea spp. in the Pacific reveals two putative novel species and the first report of dioscorea bacilliform RT virus 2Virus Research, 2017
- The genome sequence of Dioscorea bacilliform TR virus, a member of the genus Badnavirus infecting Dioscorea spp., sheds light on the possible function of endogenous Dioscorea bacilliform virusesArchiv für die gesamte Virusforschung, 2016
- Complete genome sequence of a putative new secovirus infecting yam (Dioscorea) plantsArchiv für die gesamte Virusforschung, 2016
- A Sequence-Independent Strategy for Amplification and Characterisation of Episomal Badnavirus Sequences Reveals Three Previously Uncharacterised Yam BadnavirusesViruses, 2016
- Consumers' preferences for fresh yam: a focus group studyFood Science & Nutrition, 2016
- Complete genome sequence of yam chlorotic necrotic mosaic virus from Dioscorea parvifloraArchiv für die gesamte Virusforschung, 2016
- Obtention de plantes indemnes du virus de la mosaïque de l'igname (YMV) par culture in vitro des apex chez l'igname américaine Dioscorea trifida LAgronomy for Sustainable Development, 1990