Development and first in‐human use of a Raman spectroscopy guidance system integrated with a brain biopsy needle
- 12 January 2019
- journal article
- research article
- Published by Wiley in Journal of Biophotonics
- Vol. 12 (3), e201800396
- https://doi.org/10.1002/jbio.201800396
Abstract
Navigation‐guided brain biopsies are the standard of care for diagnosis of several brain pathologies. However, imprecise targeting and tissue heterogeneity often hinder obtaining high‐quality tissue samples, resulting in poor diagnostic yield. We report the development and first clinical testing of a navigation‐guided fiberoptic Raman probe that allows surgeons to interrogate brain tissue in situ at the tip of the biopsy needle, prior to tissue removal. The 900μm diameter probe can detect high spectral quality Raman signals in both the fingerprint and high wavenumber spectral regions with minimal disruption to the neurosurgical workflow. The probe was tested in 3 brain tumor patients, and the acquired spectra in both normal brain and tumor tissue demonstrated the expected spectral features, indicating the quality of the data. As a proof‐of‐concept, we also demonstrate the consistency of the acquired Raman signal with different systems and experimental settings. Additional clinical development is planned to further evaluate the performance of the system and develop a statistical model for real‐time tissue classification during the biopsy procedure.Keywords
Funding Information
- Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
- Medtronic
- Mitacs
- Natural Sciences and Engineering Research Council of Canada
This publication has 44 references indexed in Scilit:
- Identification of Pediatric Brain Neoplasms Using Raman SpectroscopyPediatric Neurosurgery, 2012
- Frameless robotically targeted stereotactic brain biopsy: feasibility, diagnostic yield, and safetyJournal of Neurosurgery, 2012
- 5-Aminolevulinic acid-induced protoporphyrin IX fluorescence as immediate intraoperative indicator to improve the safety of malignant or high-grade brain tumor diagnosis in frameless stereotactic biopsiesActa Neurochirurgica, 2012
- Towards improving the safety and diagnostic yield of stereotactic biopsy in a single centreActa Neurochirurgica, 2010
- Image-guided frameless stereotactic biopsy without intraoperative neuropathological examinationJournal of Neurosurgery, 2010
- Stereotactic brain biopsy: Single center retrospective analysis of complicationsClinical Neurology and Neurosurgery, 2009
- Automated Autofluorescence Background Subtraction Algorithm for Biomedical Raman SpectroscopyApplied Spectroscopy, 2007
- Raman Spectroscopic Characterization of Porcine Brain Tissue Using a Single Fiber-Optic ProbeAnalytical Chemistry, 2006
- Fiber-Optic Probes for in Vivo Raman Spectroscopy in the High-Wavenumber RegionAnalytical Chemistry, 2005
- Near infrared Raman spectra of human brain lipidsSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2005