Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis
Open Access
- 19 March 2020
- journal article
- research article
- Published by MDPI AG in Journal of Clinical Medicine
- Vol. 9 (3), 854
- https://doi.org/10.3390/jcm9030854
Abstract
New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection.This publication has 32 references indexed in Scilit:
- Antibacterial Effectiveness of 2 Root Canal Irrigants in Root-filled Teeth with Infection: A Randomized Clinical TrialJournal of Endodontics, 2016
- RNA-based Assay Demonstrated Enterococcus faecalis Metabolic Activity after Chemomechanical ProceduresJournal of Endodontics, 2015
- Comparison of the Effect of Two Endodontic Irrigation Protocols on the Elimination of Bacteria from Root Canal System: A Prospective, Randomized Clinical TrialJournal of Endodontics, 2012
- DNA Binding to Hydroxyapatite: A Potential Mechanism for Preservation of Microbial DNAJournal of Endodontics, 2012
- Bacterial diversity, community structure and potential growth rates along an estuarine salinity gradientThe ISME Journal, 2012
- Optimising single‐visit disinfection with supplementary approaches: A quest for predictabilityAustralian Endodontic Journal, 2011
- Response to alkaline stress by root canal bacteria in biofilmsInternational Endodontic Journal, 2007
- Bacterial reduction and persistence after endodontic treatment proceduresOral Microbiology and Immunology, 2007
- In vivo evaluation of microbial reduction after chemo‐mechanical preparation of human root canals containing necrotic pulp tissueInternational Endodontic Journal, 2006
- Ribosomal RNA: Nature's own polymerase-amplified target for diagnosisParasitology Today, 1990