Vertical distribution of prokaryotes communities and predicted metabolic pathways in New Zealand wetlands, and potential for environmental DNA indicators of wetland condition
Open Access
- 6 January 2021
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 16 (1), e0243363
- https://doi.org/10.1371/journal.pone.0243363
Abstract
Globally, wetlands are in decline due to anthropogenic modification and climate change. Knowledge about the spatial distribution of biodiversity and biological processes within wetlands provides essential baseline data for predicting and mitigating the effects of present and future environmental change on these critical ecosystems. To explore the potential for environmental DNA (eDNA) to provide such insights, we used 16S rRNA metabarcoding to characterise prokaryote communities and predict the distribution of prokaryote metabolic pathways in peats and sediments up to 4m below the surface across seven New Zealand wetlands. Our results reveal distinct vertical structuring of prokaryote communities and metabolic pathways in these wetlands. We also find evidence for differences in the relative abundance of certain metabolic pathways that may correspond to the degree of anthropogenic modification the wetlands have experienced. These patterns, specifically those for pathways related to aerobic respiration and the carbon cycle, can be explained predominantly by the expected effects of wetland drainage. Our study demonstrates that eDNA has the potential to be an important new tool for the assessment and monitoring of wetland health.Funding Information
- New Zealand Ministry for Business, Innovation and Employment (C09X1616)
- New Zealand Ministry for Business, Innovation and Employment (C09X1527)
This publication has 44 references indexed in Scilit:
- Microbial communities in natural and disturbed peatlands: A reviewSoil Biology and Biochemistry, 2013
- Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental ScaleApplied and Environmental Microbiology, 2009
- Environmental and anthropogenic controls over bacterial communities in wetland soilsProceedings of the National Academy of Sciences of the United States of America, 2008
- High unique diversity of sulfate‐reducing prokaryotes characterized in a depth gradient in an acidic fenEnvironmental Microbiology, 2007
- Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARBApplied and Environmental Microbiology, 2006
- Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USAEnvironmental Microbiology, 2006
- Global Climate Change and Its Impact on Wetlands and Waterbird PopulationsActa Zoologica Lituanica, 2005
- Spatial patterns of aerobic limit depth and oxygen diffusion rate at two peatlands drained for forestry in AlbertaCanadian Journal of Forest Research, 1999
- Carbon dioxide and methane fluxes from drained peat soils, southern QuebecGlobal Biogeochemical Cycles, 1993
- Methane flux: Water table relations in northern wetlandsGeophysical Research Letters, 1993