In-vivo sub-diffraction adaptive optics imaging of photoreceptors in the human eye with annular pupil illumination and sub-Airy detection
- 11 March 2021
- journal article
- Published by Optica Publishing Group in Optica
- Vol. 8 (3), 333-343
- https://doi.org/10.1364/optica.414206
Abstract
Adaptive optics scanning light ophthalmoscopy (AOSLO) allows non-invasive visualization of the living human eye at the microscopic scale; but even with correction of the ocular wavefront aberrations over a large pupil, the smallest cells in the photoreceptor mosaic cannot always be resolved. Here, we synergistically combine annular pupil illumination with sub-Airy disk confocal detection to demonstrate a 33% improvement in transverse resolution (from 2.36 to 1.58 μm) and a 13% axial resolution enhancement (from 37 to 32 μm), an important step towards the study of the complete photoreceptor mosaic in heath and disease. Interestingly, annular pupil illumination also enhanced the visualization of the photoreceptor mosaic in non-confocal detection schemes such as split detection AOSLO, providing a strategy for enhanced multimodal imaging of the cone and rod photoreceptor mosaic.Keywords
Funding Information
- Research to Prevent Blindness (Departmental Award)
- Glaucoma Research Foundation (Catalyst for a Cure Initiative)
- Alcon Research Institute
- National Institutes of Health (Intramural Research Program, R01 EY025231, U01 EY025477)
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