Dengue virus strain 2 capsid protein switches the annealing pathway and reduces intrinsic dynamics of the conserved 5' untranslated region
Open Access
- 4 May 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in RNA Biology
- Vol. 18 (5), 718-731
- https://doi.org/10.1080/15476286.2020.1860581
Abstract
The capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid structural rearrangements using chaperone activity. However, the role of DENV2C during the interaction of RNA elements in the conserved 5' untranslated region (5'UTR) to the 3' untranslated region (3'UTR) is still unclear. Thus, we investigated the effect of DENV2C on the annealing mechanism of two RNA hairpin elements from the 5'UTR to their complementary sequences during (+)/(-) ds-RNAformation and (+) RNA circularization. DENV2C was found to switch the annealing pathway for RNA elements involved in (+)/(-) ds-RNA formation, but not for RNA elements related to (+) RNA circularization. In addition, we also determined that DENV2C modulates intrinsic dynamics and reduces kinetically trapped unfavourable conformations of the 5'UTR sequence. Thus, our results provide mechanistic insights by which DENV2C chaperones the interactions between RNA elements at the 5' and 3' ends during genome recombination, a prerequisite for DENV replication.Funding Information
- National Research Foundation, Singapore (NRF-CRP19-2017-03-00)
- NUS Graduate School for Integrative Sciences and Engineering
- National Research Foundation Singapore (NRF-CRP19-2017-03-00)
This publication has 65 references indexed in Scilit:
- Analysis of the RNA chaperoning activity of the hepatitis C virus core protein on the conserved 3′X region of the viral genomeNucleic Acids Research, 2011
- RNA Sequences and Structures Required for the Recruitment and Activity of the Dengue Virus PolymeraseOnline Journal of Public Health Informatics, 2011
- Kinetic analysis of the nucleic acid chaperone activity of the Hepatitis C virus core proteinNucleic Acids Research, 2010
- Dengue Virus Pathogenesis: an Integrated ViewClinical Microbiology Reviews, 2009
- HIV-1 Nucleocapsid Protein Switches the Pathway of Transactivation Response Element RNA/DNA Annealing from Loop–Loop “Kissing” to “Zipper”Journal of Molecular Biology, 2009
- Retroviral Nucleocapsid Proteins Display Nonequivalent Levels of Nucleic Acid Chaperone ActivityJournal of Virology, 2008
- The capsid-coding region hairpin element (cHP) is a critical determinant of dengue virus and West Nile virus RNA synthesisVirology, 2008
- Specific requirements for elements of the 5′ and 3′ terminal regions in flavivirus RNA synthesis and viral replicationVirology, 2008
- RNA chaperoning and intrinsic disorder in the core proteins of FlaviviridaeNucleic Acids Research, 2007
- A 5′ RNA element promotes dengue virus RNA synthesis on a circular genomeGenes & Development, 2006