Staphylococcus epidermidis Has Growth Phase Dependent Affinity for Fibrinogen and Resulting Fibrin Clot Elasticity
Open Access
- 16 June 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
Abstract
Bacterial infection and thrombosis are highly correlated, especially in patients with indwelling medical devices. Coagulase-negative staphylococci, typified by Staphylococcus epidermidis, are a common cause of medical device infections owing to their biofilm forming capacity which provides protection from antibiotics and host immune response. Attention has been drawn to the interaction between S. epidermidis and host proteins, specifically fibrinogen. However, little is known regarding the impact of the transition from planktonic to biofilm forming phenotype on this interaction. Here we investigate the growth phase dependence of bacteria-fibrinogen interaction and the resulting effect on fibrin clot formation, structure, and mechanics. Flow cytometry demonstrated growth phase dependent affinity for fibrinogen. To mimic intravascular device seeding, we quantified the adhesion of S. epidermidis to a fibrinogen coated surface under continuous flow conditions in vitro. The bacterial deposition rate onto fibrinogen was significantly greater for stationary (5,360 ± 1,776 cells/cm2s) versus exponential phase (2,212 ± 264, cells/cm2 s). Furthermore, the expression of sdrG–a cell surface adhesion protein with specificity for fibrinogen–was upregulated ∼twofold in the stationary versus the exponential phase. Rheometry and confocal microscopy demonstrated that stationary phase S. epidermidis slows clot formation and generates a more heterogeneous fibrin network structure with greater elasticity (G′ = 5.7 ± 1.0 Pa) compared to sterile fibrinogen (G′ = l.5 ± 0.2 Pa), while exponential phase cells had little effect. This work contributes to the current understanding of the growth phase dependent regulation of bacterial virulence factors and the correlation between bacterial infection and thrombosis.Funding Information
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases
This publication has 48 references indexed in Scilit:
- Evidence for persisters in Staphylococcus epidermidis RP62a planktonic cultures and biofilmsJournal of Medical Microbiology, 2011
- The effects of shear stress on isolated receptor–ligand interactions of Staphylococcus epidermidis and human plasma fibrinogen using molecularly patterned microfluidicsLab on a Chip, 2011
- Structural Hierarchy Governs Fibrin Gel MechanicsBiophysical Journal, 2010
- Staphylococcus epidermidis — the 'accidental' pathogenNature Reviews Microbiology, 2009
- Pathogenic Implication of a Fibrinogen-Binding Protein of Staphylococcus epidermidis in a Rat Model of Intravascular-Catheter-Associated InfectionInfection and Immunity, 2007
- Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidisMicrobiology, 2005
- Genomewide Analysis of Gene Expression inStaphylococcus epidermidisBiofilms: Insights into the Pathophysiology ofS. epidermidisBiofilms and the Role of Phenol‐Soluble Modulins in Formation of BiofilmsThe Journal of Infectious Diseases, 2005
- The Fibrinogen Binding Protein of Staphylococcus epidermidis Is a Target for Opsonic AntibodiesInfection and Immunity, 2004
- Clinical and Biochemical Analysis in Infection-Associated StrokeStroke, 1995
- Surface characteristics and adhesion of Escherichia coli and Staphylococcus epidermidisJournal of Applied Bacteriology, 1991