De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes
Open Access
- 13 July 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 12, 627099
- https://doi.org/10.3389/fmicb.2021.627099
Abstract
Polysaccharides separated from Lentinula edodes are well known for their medicinal properties. However, the precise molecular mechanisms of polysaccharide biosynthesis in L. edodes remain unclear. In this study, the fruiting bodies of L. edodes in four developmental stages with significant differences in polysaccharide yield were collected, and the characteristics of polysaccharides were studied. De novo sequencing and comparative transcriptomic analysis were performed by using high-throughput Illumina RNA-sequencing. KS1P30, KS2P30, KS3P30, and KS4P30 were obtained from the four developmental stages, respectively, by hot water extraction and 30% ethanol precipitation. These four polysaccharides had good immune activity in vitro; all of them were β-glucopyranose with a high molecular weight. Glucose was the main monosaccharide component of these polysaccharides. High-quality clean reads (57.88, 53.17, 53.28, and 47.56 million for different growth stages) and mapping ratios ranging from 84.75 to 90.11% were obtained. In total, 11,493 (96.56%) unigenes and 18,924 (97.46%) transcripts were successfully annotated in five public databases. The biosynthetic pathway and related genes of LEFP30 were mined. The molecular mechanism of LEFP30 yield change in the different developmental stages was predicted. The results provide some insights into the possible mechanisms involved in the biosynthetic pathway of this kind of polysaccharide in L. edodes fruiting bodies. They also indicate that candidate genes can be used as important resources for biotechnology and molecular breeding to regulate L. edodes fruiting body polysaccharide biosynthesis.This publication has 69 references indexed in Scilit:
- Genome sequence of the model medicinal mushroom Ganoderma lucidumNature Communications, 2012
- Effects of Management Factors on the Concentration of a High Molecular Weight Polysaccharide Fraction from Log-Grown Shiitake Mushrooms (Lentinula edodes (Berk.) Pegler)Journal of Agricultural and Food Chemistry, 2010
- Lentinus edodes heterogalactan: Antinociceptive and anti-inflammatory effectsFood Chemistry, 2008
- Anomalous behavior at the I2/a to Imab phase transition in SiO2-moganite: An analysis using hard-mode Raman spectroscopyAmerican Mineralogist: Journal of Earth and Planetary Materials, 2007
- Effects of molecular structure on antitumor activities of (1→3)-β-d-glucans from different Lentinus EdodesCarbohydrate Polymers, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- FT-IR study of plant cell wall model compounds: pectic polysaccharides and hemicellulosesCarbohydrate Polymers, 2000
- The effect of molecular weight and β‐1,6‐linkages on priming of macrophage function in mice by (1,3)‐β‐d‐glucanImmunology & Cell Biology, 1999
- Activity of 1,3-β-D-Glucan Synthase Requires the GTP-binding Protein Rho1Online Journal of Public Health Informatics, 1996
- EditorialRevue Française de Transfusion et Immuno-hématologie, 1986