BACH2 enforces the transcriptional and epigenetic programs of stem-like CD8+ T cells
- 11 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Immunology
- Vol. 22 (3), 370-380
- https://doi.org/10.1038/s41590-021-00868-7
Abstract
During chronic infection and cancer, a self-renewing CD8+ T cell subset maintains long-term immunity and is critical to the effectiveness of immunotherapy. These stem-like CD8+ T cells diverge from other CD8+ subsets early after chronic viral infection. However, pathways guarding stem-like CD8+ T cells against terminal exhaustion remain unclear. Here, we show that the gene encoding transcriptional repressor BACH2 is transcriptionally and epigenetically active in stem-like CD8+ T cells but not terminally exhausted cells early after infection. BACH2 overexpression enforced stem-like cell fate, whereas BACH2 deficiency impaired stem-like CD8+ T cell differentiation. Single-cell transcriptomic and epigenomic approaches revealed that BACH2 established the transcriptional and epigenetic programs of stem-like CD8+ T cells. In addition, BACH2 suppressed the molecular program driving terminal exhaustion through transcriptional repression and epigenetic silencing. Thus, our study reveals a new pathway that enforces commitment to stem-like CD8+ lineage and prevents an alternative terminally exhausted cell fate.Keywords
Funding Information
- U.S. Department of Health & Human Services | NIH | Office of Extramural Research, National Institutes of Health (AG056524, AI105343, AI117950, AI082630, AI112521, AI115712, AI108545, CA210944, CA234842)
- U.S. Department of Health & Human Services | National Institutes of Health (1ZIA DK075149 01, 1ZIA BC011480 07, 1ZIA NS003111 11, 1ZIA NS003112 11, 1ZIA AR041159 12, 1ZIA AR041167 12, 1ZIA AR041106 25, 1ZIA AI001241 01, 1ZIA AI001240 01)
- NIH Office of Dietary Supplements
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