Antiviral, Antioxidant, and Antihemolytic Effect of Annona muricata L. Leaves Extracts
Open Access
- 25 November 2020
- Vol. 9 (12), 1650
- https://doi.org/10.3390/plants9121650
Abstract
Annona muricata L. is a tropical tree that is used in traditional medicine around the world. The high content of flavonoid, alkaloid, acetogenin, phenolic and lipophilic compounds of this tropical tree forms the basis of its traditional medical uses. Our objective was to study soursop leaf extracts to support their use as antiviral therapies and investigate their protective effects against oxidative damage. The aqueous extract (AE) and acidified ethanolic extract (AEE) of soursop leaves were characterized by ultra performance liquid chromatography (UPLC), and their effects on human erythrocytes and in vitro antioxidant capacity, as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays, were investigated. The antiviral effects were evaluated using a bacteriophage surrogate. AEE showed the highest phenolic content, with rutin as the predominant compound. This extract showed higher values in the DPPH and ABTS assays, with 23.61 ± 0.42 and 24.91 ± 0.16 mmol of Trolox equivalent per gram, respectively. Inhibition of hemolysis was 34% and 51% for AE and AEE, respectively. AEE was selected for the antiviral study because of its higher antioxidant activity. The viral reduction ranged from 5–6 log10 plaque-forming units/volume (PFU) at contact times of 15–360 min. Soursop leaves have a positive effect on reducing oxidative stress in human erythrocytes and viral infections.Keywords
This publication has 30 references indexed in Scilit:
- Extracts from Annona Muricata L. and Annona Reticulata L. (Annonaceae) Potently and Selectively Inhibit Plasmodium FalciparumMedicines, 2015
- Annonaa muricata Linn Leaf Induce Apoptosis in Cancer Cause VirusJournal of Cancer Therapy, 2013
- Interaction between plant polyphenols and the erythrocyte membraneCellular & Molecular Biology Letters, 2012
- Characterization of bacteriophages with a lytic effect on various Salmonella serotypes and Escherichia coli O157:H7Canadian Journal of Microbiology, 2011
- Kaempferol inhibits enterovirus 71 replication and internal ribosome entry site (IRES) activity through FUBP and HNRP proteinsFood Chemistry, 2011
- Protective effects of Annona muricata linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic ratsAfrican Journal of Traditional, Complementary and Alternative Medicines, 2010
- Flavonoids from the leaves of Actinidia kolomiktaChemistry of Natural Compounds, 2010
- Aplicación de diversos métodos químicos para determinar actividad antioxidante en pulpa de frutosFood Science and Technology, 2005
- Morphological, Host Range, and Genetic Characterization of Two ColiphagesApplied and Environmental Microbiology, 2003
- Cheritamine, A New N‐Fatty Acyl Tryptamine and Other Constituents from the Stems of Annona cherimolaJournal of the Chinese Chemical Society, 1999