CD5L, Macrophage Apoptosis Inhibitor, Was Identified in Epicardial Fat-Secretome and Regulated by Isoproterenol From Patients With Heart Failure
Open Access
- 30 June 2020
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Physiology
- Vol. 11, 620
- https://doi.org/10.3389/fphys.2020.00620
Abstract
Objectives Neurohormonal dysfunction, which can regulate epicardial fat activity, is one of the main promoters of atrial fibrillation (AF) in patients with heart failure (HF). Our aim was to study the epicardial fat mediators for AF in patients with HF and its catecholaminergic regulation. Methods We have included 29 patients with HF who underwent cardiac surgery and were followed up for 5 years. Released proteins by epicardial adipose tissue (EAT) after isoproterenol treatment were identified by nano-high-performance liquid chromatography (HPLC) and triple time-of-flight (TOF) analysis. Common and differential identified proteins in groups of patients with AF before and after surgery were determined by the FunRich tool. Plasma and epicardial fat biopsy proteins were quantified by western blot. Results Our results identified 17 common released proteins by EAT, after isoproterenol treatment, from HF patients who suffered AF or developed new-onset AF during follow-up. Mostly, they were involved on inflammatory response and extracellular matrix. One of them was CD5L, a macrophage apoptosis inhibitor. Its secretion by isoproterenol treatment was validated on western blot. The CD5L levels on epicardial fat were also higher in the group of male patients who present or develop AF (0.44 +/- 0.05 vs. 0.18 +/- 0.15;p< 0.016). However, there were no differences regarding plasma levels. Conclusion Our results suggest the role of epicardial fat CD5L as a mediator of AF and its possible paracrine effect by catecholaminergic activity.This publication has 33 references indexed in Scilit:
- High-resolution two-dimensional gel electrophoresis analysis of atrial tissue proteome reveals down-regulation of fibulin-1 in atrial fibrillationInternational Journal of Cardiology, 2011
- Total and Interatrial Epicardial Adipose Tissues Are Independently Associated With Left Atrial Remodeling in Patients With Atrial FibrillationJournal of Cardiovascular Electrophysiology, 2011
- Pericardial Fat Is Associated With Prevalent Atrial FibrillationCirculation: Arrhythmia and Electrophysiology, 2010
- The Sympathetic Nervous System in Heart Failure: Physiology, Pathophysiology, and Clinical ImplicationsJournal of the American College of Cardiology, 2009
- Relation of Obesity to Atrial Fibrillation After Isolated Coronary Artery Bypass GraftingThe American Journal of Cardiology, 2009
- Nonlinear Fitting Method for Determining Local False Discovery Rates from Decoy Database SearchesJournal of Proteome Research, 2008
- Extracellular Matrix Alterations in Patients With Paroxysmal and Persistent Atrial Fibrillation: Biochemical Assessment of Collagen Type-I TurnoverJournal of the American College of Cardiology, 2008
- The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass SpectraMolecular & Cellular Proteomics, 2007
- Relationship of epicardial adipose tissue with atrial dimensions and diastolic function in morbidly obese subjectsInternational Journal of Cardiology, 2007
- Obesity and the Risk of Heart FailureThe New England Journal of Medicine, 2002