UPGMA - analysis of type II CRISPR RNA-guided endonuclease Cas9 homologues from the compost metagenome
Open Access
- 3 June 2021
- journal article
- research article
- Published by EDP Sciences in E3S Web of Conferences
- Vol. 265, 04010
- https://doi.org/10.1051/e3sconf/202126504010
Abstract
Metagenomic approaches provide access to the genetic diversity of the environment for biotechnological applications, allowing the discovery of new enzymes and new pathways for numerous catalytic processes. Five new putative type II CRISPR-Cas9 DNA endonucleases were identified from the compost community using the DELTA-BLAST algorithm. It was determined using phylogenetic UPGMA analysis that four of these potential enzymes are similar to those of the Bacteroidetes. Protein structural modeling confirmed the data of DELTA-BLAST and UPGMA analysis. These new five proteins found may be promising for genome editing in termoresistant Actinomyces.This publication has 12 references indexed in Scilit:
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