Can DNA barcoding be used to identify closely related Clunio Haliday, 1855 species (Diptera: Chironomidae, Orthocladiinae)?
- 11 February 2021
- journal article
- research article
- Published by Magnolia Press in Zootaxa
- Vol. 4927 (1), 1-8
- https://doi.org/10.11646/zootaxa.4927.1.1
Abstract
DNA barcoding based on a fragment of mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) was applied to the two chironomids Clunio balticus Heimbach (690 base pairs) and C. ponticus Michailova (691 base pairs). The two species differed by one deletion in the nucleotide sequence Adenine. However, the 658-nucleotide long sequences of DNA from the mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) of C. balticus and C. ponticus were identical upon comparison. Further, they compared with homologous sequences for C. marinus Holiday and C. tsushimensis Tokunaga from the Barcode of Life (BOLD) database and the results plotted as a weighted graph, where C. tsushimensis, C. marinus and C. balticus C. ponticus formed three almost equidistant groups. From this, we established that the genetic distance between the respective COI sequences of C. balticus and C. ponticus is minimal, indicating a close relationship between the species indicative of recent common origin. However, the comparative analysis between C. tsushimensis, C. marinus, C. balticus and C. ponticus showed a wider divergence in their respective nucleotide sequences. Overall, our results emphasized that the COI region does not work well as a DNA barcode to identify species within the Clunio genus. Either longer sequences or a multifaceted methodological approach, including morphology, cytogenetic and ecology is needed to distinguish some members of Clunio genus.Keywords
This publication has 19 references indexed in Scilit:
- DNA barcodes for ecology, evolution, and conservationTrends in Ecology & Evolution, 2015
- Analysis Tool Web Services from the EMBL-EBINucleic Acids Research, 2013
- First Large-Scale DNA Barcoding Assessment of Reptiles in the Biodiversity Hotspot of Madagascar, Based on Newly Designed COI PrimersPLOS ONE, 2012
- Current progress in DNA barcoding and future implications for entomologyEntomological Science, 2011
- Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal OmegaMolecular Systems Biology, 2011
- Strong genetic differentiation and postglacial origin of populations in the marine midge Clunio marinus (Chironomidae, Diptera)Molecular Ecology, 2010
- DNA barcode divergence among species and genera of birds and fishesMolecular Ecology Resources, 2009
- Identifying Canadian Freshwater Fishes through DNA BarcodesPLOS ONE, 2008
- A comprehensive DNA sequence library is essential for identification with DNA barcodesMolecular Phylogenetics and Evolution, 2007
- Biological identifications through DNA barcodesProceedings Of The Royal Society B-Biological Sciences, 2003