The Inhibitory Effects of Gold Nanoparticles on VEGF-A-Induced Cell Migration in Choroid-Retina Endothelial Cells
Open Access
- 23 December 2019
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (1), 109
- https://doi.org/10.3390/ijms21010109
Abstract
Background: Vascular endothelial growth factor (VEGF) is upregulated by hypoxia and is a crucial stimulator for choroidal neovascularization (CNV) in age-related macular degeneration and pathologic myopia, as well as retinal neovascularization in proliferative diabetic retinopathy. Retinal and choroidal endothelial cells play key roles in the development of retinal and CNV, and subsequent fibrosis. At present, the effects of gold nanoparticles (AuNPs) on the VEGF-induced choroid-retina endothelial (RF/6A) cells are still unknown. In our study, we investigated the effects of AuNPs on RF/6A cell viabilities and cell adhesion to fibronectin, a major ECM protein of fibrovascular membrane. Furthermore, the inhibitory effects of AuNPs on RF/6A cell migration induced by VEGF and its signaling were studied. Methods: The cell viability assay was used to determine the viability of cells treated with AuNPs. The migration of RF/6A cells was assessed by the Transwell migration assay. The cell adhesion to fibronectin was examined by an adhesion assay. The VEGF-induced signaling pathways were determined by western blotting. Results: The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay revealed no cytotoxicity of AuNPs on RF/6A cells. AuNPs inhibited VEGF-induced RF/6A cell migration in a concentration-dependent manner but showed no significant effects on RF/6A cell adhesion to fibronectin. Inhibitory effects of AuNPs on VEGF-induced Akt/eNOS were found. Conclusions: These results suggest that AuNPs are an effective inhibitor of VEGF-induced RF/6A cell migration through the Akt/eNOS pathways, but they have no effects on their cell viabilities and cell adhesion to fibronectin.Funding Information
- Cardinal Tien Hospital (CTH-102-1-2A30, CTH-104-1-2B05)
This publication has 56 references indexed in Scilit:
- Inhibition effects of gold nanoparticles on proliferation and migration in hepatic carcinoma-conditioned HUVECsBioorganic & Medicinal Chemistry Letters, 2014
- Mechanistic study on the biological effects of silver and gold nanoparticles in Caco-2 cells – Induction of the Nrf2/HO-1 pathway by high concentrations of silver nanoparticlesToxicology Letters, 2014
- A systems biology view of blood vessel growth and remodellingJournal of Cellular and Molecular Medicine, 2013
- Inhibitory Effects of Resveratrol on PDGF-BB-Induced Retinal Pigment Epithelial Cell Migration via PDGFRβ, PI3K/Akt and MAPK pathwaysPLOS ONE, 2013
- Effects of Gold Nanoparticles on Endotoxin-Induced Uveitis in RatsInvestigative Ophthalmology & Visual Science, 2012
- Gold Nanoparticles Downregulate Interleukin‐1β‐Induced Pro‐Inflammatory ResponsesSmall, 2012
- Serum autoantibody biomarkers for age-related macular degeneration and possible regulators of neovascularizationExperimental and Molecular Pathology, 2012
- AngiogenesisCold Spring Harbor Perspectives in Biology, 2011
- Cytotoxicity and Immunological Response of Gold and Silver Nanoparticles of Different SizesSmall, 2009
- Endothelial Cell Migration During AngiogenesisCirculation Research, 2007