Escherichia coli O8:H8 Carrying a Novel Variant of the Heat-Labile Enterotoxin LT2 Gene Caused Outbreaks of Diarrhea
Open Access
- 1 January 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in Open Forum Infectious Diseases
- Vol. 7 (1), ofaa021
- https://doi.org/10.1093/ofid/ofaa021
Abstract
No outbreaks caused by Escherichia coli–producing heat-labile enterotoxin LT2 have been reported to date. Here, we revealed that the E. coli O8:H8 strains isolated from patients in 2 independent diarrhea outbreaks were negative for any known virulence determinants in routine microbiological tests, were very closely related, and carried a prophage-encoded gene for a novel LT2 variant (LT2d) and the genes for colonization factor antigen III. We also showed that LT2d has a cytotonic activity similar to LT1. These data indicate the importance of E. coli strains producing LT2d as a human pathogen.Funding Information
- JSPS KAKENHI (16H06279, 17H04077, 16H05190)
- AMED (JP17efk0108127h0002, 18fk0108065h0801)
This publication has 22 references indexed in Scilit:
- Allele Variants of Enterotoxigenic Escherichia coli Heat-Labile Toxin Are Globally Transmitted and Associated with Colonization FactorsJournal of Bacteriology, 2014
- Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distributionNature Genetics, 2014
- Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short readsGenome Research, 2014
- Recent Advances in Understanding Enteric Pathogenic Escherichia coliClinical Microbiology Reviews, 2013
- Type II Heat-Labile Enterotoxins from 50 Diverse Escherichia coli Isolates Belong Almost Exclusively to the LT-IIc Family and May Be Prophage EncodedPLOS ONE, 2012
- Phage regulatory circuits and virulence gene expressionCurrent Opinion in Microbiology, 2005
- VFDB: a reference database for bacterial virulence factorsNucleic Acids Research, 2004
- One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsProceedings of the National Academy of Sciences of the United States of America, 2000
- Colonization factors of human enterotoxigenic Escherichia coli (ETEC)Trends in Microbiology, 1996
- Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capabilityNucleic Acids Research, 1990