Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches
Open Access
- 14 May 2021
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 7 (20), eabf0677
- https://doi.org/10.1126/sciadv.abf0677
Abstract
Methods capable of maintaining gut microbiota homeostasis to prevent bacterial translocation and infection under external threats are critical for multiple facets of human health but have been rarely reported. Here, we describe the elicitation of mucosal immunity to modulate the gut microbiota by oral delivery of living probiotics into Peyer’s patches. Probiotics are individually camouflaged within a yeast membrane, on which the embedded β-glucan can facilitate the phagocytosis of microfold cells that locate in the intestinal epithelium. The delivery of probiotics into lymphoid follicles after oral ingestion promotes robust mucosal immune responses and notably upgrades the production of secretory immunoglobulin A. The provoked immunity positively regulates the gut microflora, which, in turn, retains gut homeostasis and provides defense against environmental attacks. In two murine models of gut barrier impairment, oral administration with camouflaged probiotics effectively prevents the breakdown of intestinal barrier and evidences limited bacterial translocation and systemic inflammation.Keywords
Funding Information
- National Natural Science Foundation of China (21875135)
This publication has 56 references indexed in Scilit:
- Fecal microbiota transplantation: effectiveness, complexities, and lingering concernsMucosal Immunology, 2014
- The Uptake of Soluble and Particulate Antigens by Epithelial Cells in the Mouse Small IntestinePLOS ONE, 2014
- Therapeutic Potential of Fecal Microbiota TransplantationGastroenterology, 2013
- Multi-Faceted Functions of Secretory IgA at Mucosal SurfacesFrontiers in Immunology, 2013
- Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cellsJournal of Visualized Experiments, 2011
- Secretory IgA's complex roles in immunity and mucosal homeostasis in the gutMucosal Immunology, 2011
- The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in ProteobacteriaPLOS ONE, 2009
- Orally delivered siRNA targeting macrophage Map4k4 suppresses systemic inflammationNature, 2009
- Evolution of Symbiotic Bacteria in the Distal Human IntestinePLoS Biology, 2007
- Development and Maintenance of the Gut-Associated Lymphoid Tissue (Galt): the Roles of Enteric Bacteria and VirusesDevelopmental Immunology, 1998