Fast non-destructive assessment of heavy metal presence by ATR–FTIR analysis of crayfish exoskeleton
- 7 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Environmental Science and Pollution Research
- Vol. 27 (17), 21021-21031
- https://doi.org/10.1007/s11356-020-08405-z
Abstract
Freshwater crayfish are bioindicators of environmental pollution, often used for the assessment of heavy metal (HM) presence in the tissues, a time-consuming and expensive task. In this study, we propose the use of the vibrational spectroscopy to detect in a fast, non-destructive and sensitive way the presence of HM in the cephalothorax exoskeleton of the freshwater crayfish. Incorporation of HM into the cephalothorax exoskeleton was investigated under controlled laboratory conditions. In particular, the cephalothorax exoskeleton of five crayfish species (Astacus leptodactylus, Procambarus clarkii, Austropotamobius pallipes, Faxonius limosus, and Pacifastacus leniusculus) was analyzed by attenuated total reflection–Fourier transformed infrared (ATR–FTIR) spectroscopy in the presence or absence of cadmium (Cd), chromium (Cr), lead (Pb), nickel (Ni), and zinc (Zn) up to 4 weeks at various concentrations (0.01, 0.1, 1, 10, ppm). The ATR–FTIR profile of the crayfish cephalothorax exoskeleton was compatible with the presence of amorphous calcium carbonate, chitin, and proteins. The incubation with the HM revealed two main modifications: the shift of the peak from 859 to 872 cm−1 and the appearance of a peak at 712 cm−1. Both are ascribable to the HM interaction with calcium carbonate. The absorbance of both peaks increased along with the time of incubation, and the HM concentration. We conclude that ATR–FTIR analysis can be a useful, quick, and cost-sensitive tool to detect HM presence in the crayfish cephalothorax exoskeleton. However, it has to be regarded as a non-specific analytical technique for assessing HM contamination, since it is unable to discriminate between different HM.Keywords
This publication has 43 references indexed in Scilit:
- Comparative Ultrastructure and Carbohydrate Composition of Gastroliths from Astacidae, Cambaridae and Parastacidae Freshwater Crayfish (Crustacea, Decapoda)Biomolecules, 2012
- Manufacturing pellets with different binders: Effect on water stability and feeding response in juvenile Cherax albidusAquaculture, 2012
- Crayfish Carapace Micro-powder (CCM): A Novel and Efficient Adsorbent for Heavy Metal Ion Removal from WastewaterWater, 2010
- Rheological behavior of polysaccharide based pellets for crayfish feeding tested on growth in the crayfish Cherax albidusAquaculture, 2008
- Biomonitoring: An appealing tool for assessment of metal pollution in the aquatic ecosystemAnalytica Chimica Acta, 2008
- DETECTION OF HEAVY METALS IN FARMED CHERAX DESTRUCTORBulletin Français de la Pêche et de la Pisciculture, 2006
- Taking Advantage of Disorder: Amorphous Calcium Carbonate and Its Roles in BiomineralizationAdvanced Materials, 2003
- A novel tridentate co-ordination mode for the carbonatonickel systemJ. Chem. Soc., Dalton Trans., 1997
- Infrared Spectra of Amorphous and Crystalline Calcium Carbonate.Acta Chemica Scandinavica, 1991
- Patterns of trace metal accumulation in crayfish populationsBulletin of Environmental Contamination and Toxicology, 1978