Monoclonal Antibodies Specific to the Extracellular Domain of Histidine Kinase YycG of Staphylococcus epidermidis Inhibit Biofilm Formation
Open Access
- 7 August 2020
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Microbiology
- Vol. 11, 1839
- https://doi.org/10.3389/fmicb.2020.01839
Abstract
Staphylococcus epidermidis is frequently associated with biofilm-related infections. Biofilms drastically reduce the efficacy of conventional antibiotics and the host immune system. In S. epidermidis biofilm formation, a major role is played by the YycG/YycF two-component system, and previous findings have indicated that inhibitors targeting the cytoplasmic HATPase_c domain of YycG kinase in S. epidermidis exhibit bactericidal and biofilm-killing activities. Therefore, we hypothesized that monoclonal antibodies (mAbs) against YycG extracellular (YycGex) domain would block the signal transduction and influence the biofilm formation of S. epidermidis. In this study, we screened out two YycGex-specific mAbs showing the highest affinity for the target, mAbs 2F3 and 1H1. These mAbs inhibited S. epidermidis biofilm formation in a dose-dependent manner, and at a concentration of 160 μg/mL, mAbs 2F3 and 1H1 caused 78.3 and 93.1% biofilm reduction, respectively, relative to normal mouse IgG control. When co-cultivated with YycGex mAbs, S. epidermidis cells showed diminished initial-adherence capacity, and the antibody treatment further led to a marked decrease in the synthesis of polysaccharide intercellular adhesin and in the transcriptional level of genes encoding proteins involved in biofilm formation. Lastly, we determined that the epitopes recognized by the two YycGex mAbs are located within aa 59–70 of the YycGex domain. It indicates that the YycGex domain may be a potential candidate as a vaccine for the prevention of S. epidermidis biofilm infections.Funding Information
- National Major Science and Technology Projects of China
- National Natural Science Foundation of China
- National Natural Science Foundation of China
- National Major Science and Technology Projects of China
This publication has 64 references indexed in Scilit:
- Reductive evolution and the loss of PDC/PAS domains from the genus StaphylococcusBMC Genomics, 2013
- Role of the SaeRS two-component regulatory system in Staphylococcus epidermidisautolysis and biofilm formationBMC Microbiology, 2011
- Impact of the Staphylococcus epidermidis LytSR two-component regulatory system on murein hydrolase activity, pyruvate utilization and global transcriptional profileBMC Microbiology, 2010
- Extracytoplasmic PAS-Like Domains Are Common in Signal Transduction ProteinsJournal of Bacteriology, 2010
- Modulation of eDNA Release and Degradation Affects Staphylococcus aureus Biofilm MaturationPLOS ONE, 2009
- A sensor histidine kinase co‐ordinates cell wall architecture with cell division in Bacillus subtilisMolecular Microbiology, 2008
- Essentiality, Bypass, and Targeting of the YycFG (VicRK) Two-Component Regulatory System in Gram-Positive BacteriaJournal of Bacteriology, 2008
- An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteinsProceedings of the National Academy of Sciences of the United States of America, 2008
- Effects of Oxygen on Biofilm Formation and the AtlA Autolysin of Streptococcus mutansJournal of Bacteriology, 2007
- The Crystal Structure of Bacillus subtilis YycI Reveals a Common Fold for Two Members of an Unusual Class of Sensor Histidine Kinase Regulatory ProteinsJournal of Bacteriology, 2007