miR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells
- 1 April 2020
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Gastrointestinal and Liver Physiology
- Vol. 318 (4), G827-G839
- https://doi.org/10.1152/ajpgi.00369.2019
Abstract
There is increasing evidence that microRNA (miRNA) abnormity is involved in the occurrence and the development of various malignancies, including colon cancer. MiRNA-524-5p has been reported to possess anti-cancer activity in various tumors, which function is seldom investigated in colon cancer cells. The aim of this study was to explore the effect of miRNA-524-5p/with-nolysine kinases 1 (WNK1) system on angiogenesis in colon cancer cell line (HT-29 and COLO205 cells) and further investigate the potential mechanisms. We found miRNA-524-5p expression was relatively high in COLO205 cells and relatively low in HT-29 cells. Elevating miRNA-524-5p expression inhibited proliferation, induced cycle arrest, diminished VEGF production, and thereby suppressing angiogenesis in HT-29 cells. WNK1 silencing exerted the ability of anti-angiogenesis in HT-29 cells. Besides, miRNA-524-5p deficiency-induced angiogenesis was impeded by WNK1 silence in COLO205 cells. In a murine tumor model, miRNA-524-5p agomir treatment significantly suppressed colon cancer tumorigenicity with the down-regulation of WNK1 expression. In summary, our results indicated that miRNA-524-5p inhibited angiogenesis in colon cancer cells via targeting WNK1.Keywords
Funding Information
- the Natural Science Foundation of Heilongjiang Province (No. H2017078)
- the Biomedicine Climbing Project for Provincial Colleges of Department of Education, Heilongjiang Province (No. 2017-KYYWFMY-0653 and 2017-KYYWFMY-0684)
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