Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry
Open Access
- 2 February 2021
- Vol. 26 (3), 764
- https://doi.org/10.3390/molecules26030764
Abstract
89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.Keywords
Funding Information
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (PP00P2_163683 and PP00P2_190093)
- Horizon 2020 Framework Programme (rant Agreement No: 676904, ERC-StG-2015, NanoSCAN; 101001734, ERC-CoG-2020, PhotoPHARMA)
- Krebsliga Schweiz (KLS-4257-08-2017)
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