A Photoactivatable Small‐Molecule Probe for the In Vivo Capture of Polyketide Intermediates
Open Access
- 16 October 2019
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 25 (72), 16511-16514
- https://doi.org/10.1002/chem.201903661
Abstract
A photolabile carba(dethia) malonyl N‐acetylcysteamine derivative was devised and prepared for the trapping of biosynthetic polyketide intermediates following light activation. From the lasalocid A polyketide assembly in a mutant strain of the soil bacterium S. lasaliensis, a previously undetected cyclised intermediate was identified and characterised, providing a new outlook on the timing of substrate processing.Funding Information
- Biotechnology and Biological Sciences Research Council (BB/J007250/1, 1231225)
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