Untargeted Metabolomics Approach Reveals Diverse Responses of Pastinaca Sativa to Ozone and Wounding Stresses
Open Access
- 23 July 2019
- journal article
- research article
- Published by MDPI AG in Metabolites
- Vol. 9 (7), 153
- https://doi.org/10.3390/metabo9070153
Abstract
Stresses such as wounding or atmospheric pollutant exposure have a significant impact on plant fitness. Since it has been widely described that the metabolome directly reflects plant physiological status, a way to assess this impact is to perform a global metabolomic analysis. In this study, we investigated the effect of two abiotic stresses (mechanical wounding and ozone exposure) on parsnip metabolic balance using a liquid chromatography-mass spectrometry-based untargeted metabolomic approach. For this purpose, parsnip leaves were submitted to an acute ozone exposure or were mechanically wounded and sampled 24, 48, and 72 h post-treatment. Multivariate and univariate statistical analyses highlighted numerous differentially-accumulated metabolic features as a function of time and treatment. Mechanical wounding led to a more differentiated response than ozone exposure. We found that the levels of coumarins and fatty acyls increased in wounded leaves, while flavonoid concentration decreased in the same conditions. These results provide an overview of metabolic destabilization through differentially-accumulated compounds and provide a better understanding of global plant metabolic changes in defense mechanisms.Keywords
This publication has 53 references indexed in Scilit:
- Identification, characterization, and expression analysis of calmodulin and calmodulin-like genes in grapevine (Vitis vinifera) reveal likely roles in stress responsesPlant Physiology and Biochemistry, 2018
- Jasmonates in plants under abiotic stresses: Crosstalk with other phytohormones mattersEnvironmental and Experimental Botany, 2018
- Understanding and improving global crop response to ozone pollutionThe Plant Journal, 2016
- Literature review: Impact of climate change on pesticide useFood Research International, 2015
- Molecular Mechanisms Regulating Rapid Stress Signaling Networks in ArabidopsisJournal of Integrative Plant Biology, 2010
- Detoxification and repair process of ozone injury: From O3 uptake to gene expression adjustmentEnvironmental Pollution, 2009
- Heat tolerance in plants: An overviewEnvironmental and Experimental Botany, 2007
- Elicitor signal transduction leading to production of plant secondary metabolitesBiotechnology Advances, 2005
- Oxidative stress and ozone: perception, signalling and responsePlant, Cell & Environment, 2005
- CDPK-mediated signalling pathways: specificity and cross-talkJournal of Experimental Botany, 2003