Tuberculosis diagnostic accuracy of stool Xpert MTB/RIF and urine AlereLAM in vulnerable children
- 17 June 2021
- journal article
- research article
- Published by European Respiratory Society (ERS) in European Respiratory Journal
- Vol. 59 (1), 2101116
- https://doi.org/10.1183/13993003.01116-2021
Abstract
Objective In chronic pancreatitis (CP), alterations in several genes have so far been described, but only small cohorts have been extensively investigated for all predisposing genes. Design 660 patients with idiopathic or hereditary CP and up to 1758 controls were enrolled. PRSS1, SPINK1 and CTRC were analysed by DNA sequencing, and cystic fibrosis transmembrane conductance regulator (CFTR) by melting curve analysis. Results Frequencies of CFTR variants p.R75Q, p.I148T, 5T-allele and p.E528E were comparable in patients and controls. We identified 103 CFTR variants, which represents a 2.7-fold risk increase (pCFTR compound heterozygous status with variant classes CF-causing severe and mild represented an OR of 16.1 (pCFTR variants in CP is less pronounced than reported previously, with ORs between 2.7 and 4.5. Only CF-causing variants reached statistical significance. Compound and trans-heterozygosity is an overt risk factor for the development of CP, but the number of CFTR compound heterozygotes in particular is rather low. In summary, the study demonstrates the complexity of genetic interactions in CP and a minor influence of CFTR alterations in CP development.Funding Information
- Médecins Sans Frontières
This publication has 35 references indexed in Scilit:
- Urine lipoarabinomannan testing for diagnosis of pulmonary tuberculosis in children: a prospective studyThe Lancet. Global Health, 2014
- Reasons for false-positive lipoarabinomannan ELISA results in a Tanzanian populationScandinavian Journal of Infectious Diseases, 2013
- Xpert MTB/RIF Testing of Stool Samples for the Diagnosis of Pulmonary Tuberculosis in ChildrenClinical Infectious Diseases, 2013
- Foul Smelling Urine in a 7-year-old Boy Caused by Aerococcus urinaeThe Pediatric Infectious Disease Journal, 2012
- Rapid Diagnosis of Pediatric Intrathoracic Tuberculosis From Stool Samples Using the Xpert MTB/RIF AssayThe Pediatric Infectious Disease Journal, 2012
- Point-of-care detection of lipoarabinomannan (LAM) in urine for diagnosis of HIV-associated tuberculosis: a state of the art reviewBMC Infectious Diseases, 2012
- Diagnostic Accuracy of a Urine Lipoarabinomannan Enzyme-Linked Immunosorbent Assay for Screening Ambulatory HIV-Infected Persons for TuberculosisJAIDS Journal of Acquired Immune Deficiency Syndromes, 2011
- Novel lipoarabinomannan-like lipoglycan (CdiLAM) contributes to the adherence of Corynebacterium diphtheriae to epithelial cellsArchiv für Mikrobiologie, 2008
- TUBERCULOSIS IN CHILDRENPrimary Care: Clinics in Office Practice, 1996