B6.Rag1 Knockout Mice Generated at the Jackson Laboratory in 2009 Show a Robust Wild-Type Hypertensive Phenotype in Response to Ang II (Angiotensin II)
- 1 April 2020
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Hypertension
- Vol. 75 (4), 1110-1116
- https://doi.org/10.1161/HYPERTENSIONAHA.119.13773
Abstract
A key finding supporting a causal role of the immune system in the pathogenesis of hypertension is the observation that RAG1 knockout mice on a C57Bl/6J background (B6.Rag1(-/)(-)), which lack functional B and T cells, develop a much milder hypertensive response to Ang II (angiotensin II) than control C57Bl/6J mice. Here, we report that we never observed any Ang II resistance of B6.Rag1(-/-) mice purchased directly from the Jackson Laboratory as early as 2009. B6.Rag1(-/-) mice displayed nearly identical blood pressure increases monitored via radiotelemetry and hypertensive end-organ damage in response to different doses of Ang II and different levels of salt intake (0.02%, 0.3%, and 3% NaCl diet). Similarly, restoration of T-cell immunity by adoptive cell transfer did not affect the blood pressure response to Ang II in B6.Rag1(-/-) mice. Full development of the hypertension-resistant phenotype in B6.Rag1(-/-) mice appears to depend on the action of yet unidentified nongenetic modifiers in addition to the absence of functional T cells.Keywords
This publication has 29 references indexed in Scilit:
- Genetic mutation of recombination activating gene 1 in Dahl salt-sensitive rats attenuates hypertension and renal damageAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2013
- Induction of pathogenic TH17 cells by inducible salt-sensing kinase SGK1Nature, 2013
- Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cellsNature, 2013
- Myeloperoxidase deficiency preserves vasomotor function in humansEuropean Heart Journal, 2011
- Mechanisms Underlying the Cerebral Microvascular Responses to Angiotensin II-Induced HypertensionMicrocirculation, 2010
- Total lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but not abdominal aortic aneurysms in apoE deficient miceAtherosclerosis, 2010
- Intrarenal angiotensin II and angiotensinogen augmentation in chronic angiotensin II-infused miceAmerican Journal of Physiology-Renal Physiology, 2008
- Stimulation of lymphocyte responses by angiotensin II promotes kidney injury in hypertensionAmerican Journal of Physiology-Renal Physiology, 2008
- Role of the T cell in the genesis of angiotensin II–induced hypertension and vascular dysfunctionThe Journal of Experimental Medicine, 2007
- Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependentThe Journal of Experimental Medicine, 2006